核糖体蛋白L7/L12是实现最佳翻译所必需的。
Ribosomal protein L7/L12 is required for optimal translation.
作者信息
Pettersson I, Kurland C G
出版信息
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4007-10. doi: 10.1073/pnas.77.7.4007.
Abstract
We have tested the performance in vitro of Escherichia coli ribosomes containing or lacking the protein L7/L12. When the experiments are performed in an optimized mixture of ions (polymix), L7/L12 is required for maximal rate of synthesis as well as for minimal missense error frequency. The results in conventional Tris/Mg2+/NH4Cl buffers are different; in these buffers, only the rate of synthesis is strongly dependent on the presence of L7/L12. In addition, we show that there is a large difference between the optimal Mg2+ concentration required for speed of translation and that for accuracy of translation in conventional buffer. These optima are very close in polymix. Finally, we show that the contribution of L7/L12 to the speed of translation is obscured in translation systems that are limited by substrates. We conclude that it is not possible to analyze details of the mechanism of translation in conventional buffers.
摘要
我们已经测试了含有或缺乏蛋白质L7/L12的大肠杆菌核糖体的体外性能。当在优化的离子混合物（多离子混合物）中进行实验时，L7/L12对于最大合成速率以及最小错义错误频率是必需的。在传统的Tris/Mg2+/NH4Cl缓冲液中的结果不同；在这些缓冲液中，只有合成速率强烈依赖于L7/L12的存在。此外，我们表明，在传统缓冲液中，翻译速度所需的最佳Mg2+浓度与翻译准确性所需的最佳Mg2+浓度之间存在很大差异。在多离子混合物中，这些最佳值非常接近。最后，我们表明，在受底物限制的翻译系统中，L7/L12对翻译速度的贡献被掩盖了。我们得出结论，在传统缓冲液中不可能分析翻译机制的细节。
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