Hale T L, Formal S B
Am J Clin Nutr. 1980 Nov;33(11 Suppl):2485-90. doi: 10.1093/ajcn/33.11.2485.
The cytotoxicity of an invasive toxigenic wild-type strain of Shigella dysenteriae 1 (3818T) was compared with that of noninvasive, toxigenic strain 38180 and hypotoxigenic strain 725. Cytolysis of HeLa or Henle 407 cells exposed to these strains was measured by release of (3H) uridine from prelabeled monolayers. HeLa cells exposed to noninvasive, toxigenic strain 38180, or to partially purified Shiga toxin were lysed only after a latent period of more than 8 hr. During this period, protein synthesis was inhibited. In contrast, Henle 407 cells that were exposed to strain 38180 or to exogenous Shiga toxin were unaffected. When either Henle 407 or HeLa cells were infected with invasive toxigenic strains, rapid lysis ensued. Quantitative microassay of cytosol toxicity showed that Shiga toxin was produced intracellularly by strain 3818T. The data suggest that cytolysis of infected mammalian cells is caused, at least in part, by intracellular Shiga toxin.
将痢疾志贺氏菌1型（3818T）的侵袭性产毒野生型菌株的细胞毒性与非侵袭性产毒菌株38180和低产毒菌株725的细胞毒性进行了比较。通过从预先标记的单层细胞中释放（3H）尿苷来测量暴露于这些菌株的HeLa或Henle 407细胞的细胞溶解情况。暴露于非侵袭性产毒菌株38180或部分纯化的志贺毒素的HeLa细胞仅在超过8小时的潜伏期后才会裂解。在此期间，蛋白质合成受到抑制。相比之下，暴露于菌株38180或外源性志贺毒素的Henle 407细胞未受影响。当Henle 407或HeLa细胞感染侵袭性产毒菌株时，会迅速发生裂解。细胞溶质毒性的定量微量测定表明，菌株3818T在细胞内产生志贺毒素。数据表明，感染的哺乳动物细胞的细胞溶解至少部分是由细胞内志贺毒素引起的。
