Dissanayake S, Ismail M M
Bull World Health Organ. 1980;58(4):655-7.
The cross-reactivity of filarial heteroantigens, the non-availability of adult Wuchereria bancrofti for antigen extraction, and the lack of structural information on purified filarial heteroantigens make the serological diagnosis of W. bancrofti infection in man a difficult problem. The use of immune sera from cattle infected with Setaria digitata when carrying out the enzyme-linked immunosorbent assay (ELISA) largely eliminated the cross-reactions with serum antibodies generally seen in subjects showing no evidence of W. bancrofti infection.
丝虫异种抗原的交叉反应性、无法获得用于抗原提取的班氏吴策线虫成虫以及缺乏纯化丝虫异种抗原的结构信息，使得人类班氏吴策线虫感染的血清学诊断成为一个难题。在进行酶联免疫吸附测定（ELISA）时，使用感染指状腹腔丝虫的牛的免疫血清，在很大程度上消除了与通常在无班氏吴策线虫感染证据的受试者血清抗体中普遍出现的交叉反应。
