Dendinger S M, Brenchley J E
J Bacteriol. 1980 Dec;144(3):1043-7. doi: 10.1128/jb.144.3.1043-1047.1980.
Mutants of Salmonella typhimurium defective in glutamate dehydrogenase activity were isolated in parent strains lacking glutamate synthase activity by localizcd mutagenesis or by a general mutagenesis combined with a cycloserine enrichment for glutamate auxotrophs. Two mutants with temperature-sensitive phenotypes had glutamate dehydrogenase activities that were more thermolabile than that of an isogenic control strain. Eight other mutants had less than 10% of the wild-type glutamate dehydrogenase activity. All the mutations were cotransducible with a Tn10 element (zed-2:Tn10) located at approximately 23 U on the S. typhimurium linkage map. These data strongly indicate that this region contains the structural gene (gdhA) for glutamate dehydrogenase.
通过定位诱变或通过一般诱变结合环丝氨酸富集谷氨酸营养缺陷型，在缺乏谷氨酸合酶活性的亲本菌株中分离出鼠伤寒沙门氏菌谷氨酸脱氢酶活性缺陷的突变体。两个具有温度敏感表型的突变体的谷氨酸脱氢酶活性比同基因对照菌株的活性对热更不稳定。其他八个突变体的野生型谷氨酸脱氢酶活性不到10%。所有突变都与位于鼠伤寒沙门氏菌连锁图谱上约23U处的Tn10元件（zed-2:Tn10）共转导。这些数据有力地表明该区域包含谷氨酸脱氢酶的结构基因（gdhA）。
