Crocker J, Curran R C
J Clin Pathol. 1981 Oct;34(10):1080-2. doi: 10.1136/jcp.34.10.1080.
Immunoglobulin (Ig) was demonstrated in paraffin sections of 12 trephine bone marrow biopsies by means of the unlabelled antibody peroxidase-antiperoxidase (PAP) method. The Ig-containing cells, which were counted with the Reichert-Jung (Kontron) MOP-AMO(3) user-controlled image-analyser, were found to constitute approximately 4.2% of all the nucleated cells in the marrow, a figure significantly higher than those reported by previous workers.
采用未标记抗体过氧化物酶-抗过氧化物酶(PAP)法,在12例环钻骨髓活检组织的石蜡切片中检测到免疫球蛋白(Ig)。使用Reichert-Jung(Kontron)MOP-AMO(3)用户控制图像分析仪对含Ig细胞进行计数,发现其约占骨髓中所有有核细胞的4.2%,这一数字显著高于以往研究人员报告的数值。