Scaroni C, Cancellieri V, Lesser M, Vernace S J, Paronetto F
J Clin Lab Immunol. 1980 Sep;4(2):115-8.
A modification of the leukocyte adherence inhibition method is described which utilizes 51Cr-labeled blood mononuclear cells placed in microwells. The test is reproducible, objective, employs approximately 2,000 cells per well, and allows multiple replicates of several antigens. With the two antigens tested, SK-SD and PPD, both increases and decreases of leukocyte adherence are observed. Serum proteins are important in the medium and inactivated AB serum gave the best results. A good correlation was observed between this leukocyte adherence test and positivity of skin testing,
本文描述了一种白细胞黏附抑制方法的改良方法，该方法利用置于微孔中的51Cr标记的血液单核细胞。该试验具有可重复性、客观性，每孔使用约2000个细胞，并允许对几种抗原进行多次重复试验。在所测试的两种抗原SK-SD和PPD中，观察到白细胞黏附既有增加也有减少。培养基中的血清蛋白很重要，灭活的AB血清效果最佳。观察到这种白细胞黏附试验与皮肤试验阳性之间有良好的相关性。
