Joel D D, Chanana A D, Chandra P
Am Rev Respir Dis. 1980 Dec;122(6):925-32. doi: 10.1164/arrd.1980.122.6.925.
The response of the lower respiratory tract to intrabronchial administration of horse red blood cells (HRBC) was studied in sheep by continuous monitoring of efferent lymph issuing from the regional caudal mediastinal lymph node. The immune response was assessed by enumerating direct and indirect plaque-forming cells (PFC) in pulmonary lymph, bronchoalveolar lavage, and blood as a function of time after immunization. Within 3 days, PFC began to appear in pulmonary lymph, reaching a peak between Days 5 and 7. During the primary immune response, PFC were not detected in either bronchoalveolar lavage or blood. A second antigenic challenge resulted in a rapid, marked increase in lymph PFC, predominantly of the indirect type. Plaque-forming cells in lung lavage and blood, although observed after a secondary challenge, were inconsistent, particularly with the lower dosages of antigen. Within the narrow range of antigen dosage used, there was a clear dose-response relationship for a lymph-borne PFC during both the primary and secondary immune response. As with PFC, a high titer of antibody in lymph preceded its appearance in serum. The results indicated that monitoring efferent lung lymph can be a very sensitive and reproducible method to assess various aspects of pulmonary immune responses. In contrast, bronchoalveolar lavage elements did not effectively reflect the status of immune reactivity in the lower respiratory tract.
通过持续监测绵羊尾侧纵隔局部淋巴结引出的淋巴液，研究了支气管内注入马红细胞（HRBC）后下呼吸道的反应。通过计数免疫后不同时间肺淋巴、支气管肺泡灌洗物和血液中的直接和间接空斑形成细胞（PFC）来评估免疫反应。3天内，PFC开始出现在肺淋巴中，在第5至7天达到峰值。在初次免疫反应期间，支气管肺泡灌洗物或血液中均未检测到PFC。再次抗原刺激导致淋巴PFC迅速、显著增加，主要为间接型。肺灌洗物和血液中的空斑形成细胞虽然在再次刺激后可观察到，但并不稳定，尤其是低剂量抗原时。在所使用的狭窄抗原剂量范围内，初次和再次免疫反应期间淋巴源性PFC均存在明显的剂量反应关系。与PFC一样，淋巴中高滴度抗体先于其在血清中出现。结果表明，监测肺引流淋巴液是评估肺免疫反应各方面的一种非常敏感且可重复的方法。相比之下，支气管肺泡灌洗成分并不能有效反映下呼吸道的免疫反应状态。
