Yamanaka N, Ishii Y, Koshiba H, Mikuni C, Ogasawara M, Kikuchi K
Cancer. 1981 Jan 15;47(2):311-8. doi: 10.1002/1097-0142(19810115)47:2<311::aid-cncr2820470217>3.0.co;2-0.
Cell surface markers of 44 cases of non-Hodgkin's lymphoma (NHL) were studied with various surface markers, especially by using antihuman B lymphocyte serum (ABS), antihuman thymocyte serum (ATS-T), and antihuman peripheral T lymphocyte serum (ALS-T), which were rendered specific for human B lymphocytes, human thymocytes, and human peripheral T lymphocytes, respectively. An immunofluorescent study with ABS, ATS-T, and ALS-T enabled us to demonstrate the histologic localization of normal or neoplastic B and T cells in preserving the original structure of lymphoid organs or tumor tissues. The proportion of cell types in NHL was B cell type 59%, T1 (ATS-T reactive) type 7%, T2 (ALS-T reactive) type 23%, and Null (non T, non B) type 11%. The relationships among cell types, histologic findings, and clinical characteristics were also investigated. Patients with T1-NHL had mediastinal tumors, which were histologically classified into "Lymphoblastic lymphoma." These facts suggest that T1-NHL may have originated in the thymus. Patients with T2-NHL showed a high incidence of skin lesions. Median survival of ten patients with T1- and T2-NHL was seven months, which was much shorter than that of B- or Null-NHL.
应用多种表面标志物，特别是抗人B淋巴细胞血清（ABS）、抗人胸腺细胞血清（ATS-T）和抗人外周血T淋巴细胞血清（ALS-T），对44例非霍奇金淋巴瘤（NHL）的细胞表面标志物进行了研究，这些血清分别对人B淋巴细胞、人胸腺细胞和人外周血T淋巴细胞具有特异性。使用ABS、ATS-T和ALS-T进行免疫荧光研究，使我们能够在保留淋巴器官或肿瘤组织原始结构的情况下，展示正常或肿瘤性B细胞和T细胞的组织学定位。NHL中细胞类型的比例为B细胞型59%、T1（ATS-T反应性）型7%、T2（ALS-T反应性）型23%、Null（非T、非B）型11%。还研究了细胞类型、组织学发现和临床特征之间的关系。T1-NHL患者有纵隔肿瘤，组织学上分类为“淋巴母细胞淋巴瘤”。这些事实表明，T1-NHL可能起源于胸腺。T2-NHL患者皮肤病变发生率高。10例T1-和T2-NHL患者的中位生存期为7个月，远短于B-或Null-NHL患者。
