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呼吸道合胞病毒多肽抗血清的制备：在酶联免疫吸附测定中的应用。

Production of antiserum to respiratory syncytial virus polypeptides: application in enzyme-linked immunosorbent assay.

作者信息

Hornsleth A, Grauballe P C, Friis B, Genner J, Pedersen I R

出版信息

J Clin Microbiol. 1981 Nov;14(5):501-9. doi: 10.1128/jcm.14.5.501-509.1981.

DOI:10.1128/jcm.14.5.501-509.1981
PMID:7031083
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC273977/
Abstract

By use of crossed immunoelectrophoresis techniques, respiratory syncytial (RS) virus-specific precipitates were produced between RS virus cellular antigen [solubilized in tris(hydroxymethyl)aminomethane-glycine buffer, pH 9] and antiserum raised in rabbits against semipurified RS virus. When these precipitates were employed as antigens for further immunizations in rabbits, antibodies (anti-RSV-precip.I) were produced which reacted with only one RS virus antigen when tested by the crossed immunoelectrophoresis technique. Precipitates obtained between RS virus cellular antigen (labeled with L-[35S]methionine) and anti-RSV-precip.I were examined by polyacrylamide gel electrophoresis, which showed that anti-RSV-precip.I precipitated RS virus polypeptides of molecular weights 28,000 to 84,000. Anti-RSV-precip.I was employed as capture antibodies in the enzyme-linked immunosorbent assay, in which RS virus cellular antigen was used as the second layer. Determination of human RS virus immunoglobulin G antibodies by this enzyme-linked immunosorbent assay technique showed a high degree of sensitivity, specificity, and reproducibility.

摘要

通过交叉免疫电泳技术，在呼吸道合胞（RS）病毒细胞抗原[溶解于pH 9的三（羟甲基）氨基甲烷 - 甘氨酸缓冲液中]与用半纯化RS病毒免疫家兔产生的抗血清之间产生了RS病毒特异性沉淀物。当将这些沉淀物用作抗原对家兔进行进一步免疫时，产生了抗体（抗RSV - precip.I），通过交叉免疫电泳技术检测，该抗体仅与一种RS病毒抗原发生反应。通过聚丙烯酰胺凝胶电泳检查了在RS病毒细胞抗原（用L - [35S]甲硫氨酸标记）与抗RSV - precip.I之间获得的沉淀物，结果表明抗RSV - precip.I沉淀了分子量为28,000至84,000的RS病毒多肽。抗RSV - precip.I被用作酶联免疫吸附测定中的捕获抗体，其中RS病毒细胞抗原用作第二层。通过这种酶联免疫吸附测定技术测定人RS病毒免疫球蛋白G抗体显示出高度的敏感性、特异性和可重复性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4437/273977/f36722b64724/jcm00160-0070-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4437/273977/ca4e1ff3c839/jcm00160-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4437/273977/b84555944db6/jcm00160-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4437/273977/8b84b6872204/jcm00160-0069-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4437/273977/df90fb542178/jcm00160-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4437/273977/f36722b64724/jcm00160-0070-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4437/273977/ca4e1ff3c839/jcm00160-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4437/273977/b84555944db6/jcm00160-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4437/273977/8b84b6872204/jcm00160-0069-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4437/273977/df90fb542178/jcm00160-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4437/273977/f36722b64724/jcm00160-0070-b.jpg

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本文引用的文献

1
Plaquing of viruses in the absence of a carbon dioxide atmosphere.在无二氧化碳环境中病毒的蚀斑形成
Appl Microbiol. 1967 Nov;15(6):1496. doi: 10.1128/am.15.6.1496-1496.1967.
2
Studies on an epidemic of keratoconjunctivitis caused by adenovirus type 8. I. Virus isolation in human amniotic cells and serological observations.关于8型腺病毒引起的流行性角结膜炎的研究。I. 人羊膜细胞中的病毒分离及血清学观察。
Acta Ophthalmol (Copenh). 1961;39:974-83. doi: 10.1111/j.1755-3768.1961.tb03517.x.
3
Purification and polypeptides of respiratory syncytial virus.
肺炎和细支气管炎的抗生素治疗。一项前瞻性随机研究。
Arch Dis Child. 1984 Nov;59(11):1038-45. doi: 10.1136/adc.59.11.1038.
4
Enzyme-linked immunoassays for the detection of microbial antigens and their antibodies.用于检测微生物抗原及其抗体的酶联免疫测定法。
Adv Appl Microbiol. 1986;31:271-92. doi: 10.1016/s0065-2164(08)70445-3.
5
Detection of respiratory syncytial virus and rotavirus by enhanced chemiluminescence enzyme-linked immunosorbent assay.通过增强化学发光酶联免疫吸附测定法检测呼吸道合胞病毒和轮状病毒。
J Clin Microbiol. 1988 Apr;26(4):630-5. doi: 10.1128/jcm.26.4.630-635.1988.
6
A rapid test for detection of respiratory syncytial virus in nasopharyngeal secretion.一种用于检测鼻咽分泌物中呼吸道合胞病毒的快速检测方法。
Eur J Clin Microbiol Infect Dis. 1990 May;9(5):356-8. doi: 10.1007/BF01973745.
呼吸道合胞病毒的纯化及多肽
Microbiol Immunol. 1980;24(4):361-4. doi: 10.1111/j.1348-0421.1980.tb02839.x.
4
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5
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J Immunol Methods. 1980;39(1-2):95-100. doi: 10.1016/0022-1759(80)90298-7.
6
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7
New enzyme immunoassays for measurement of influenza A/Victoria/3/75 virus in nasal washes.用于检测鼻洗液中甲型/维多利亚/3/75流感病毒的新型酶免疫测定法。
Lancet. 1980 Apr 19;1(8173):851-3. doi: 10.1016/s0140-6736(80)91356-2.
8
A simple colorimetric method for determination of protein.一种测定蛋白质的简单比色法。
Anal Biochem. 1969 Oct 1;31(1):146-8. doi: 10.1016/0003-2697(69)90251-6.
9
Growth of respiratory syncytial (RS) virus in HEp-2 cell cultures. Difference in effect of proflavine and actinomycin-D.呼吸道合胞（RS）病毒在人喉表皮样癌细胞（HEp-2）培养物中的生长。原黄素与放线菌素D的作用差异。
Acta Pathol Microbiol Scand. 1969;76(4):637-45. doi: 10.1111/j.1699-0463.1969.tb03295.x.
10
Separation and concentration of respiratory syncytial virus (RSV) antigens.呼吸道合胞病毒（RSV）抗原的分离与浓缩
J Immunol Methods. 1974 Mar;4(2):349-57. doi: 10.1016/0022-1759(74)90076-3.