通过酶联免疫吸附试验抑制法检测鼻咽分泌物中的呼吸道合胞病毒。
Detection of respiratory syncytial virus in nasopharyngeal secretions by inhibition of enzyme-linked immunosorbent assay.
作者信息
Hornsleth A, Brenøe E, Friis B, Knudsen F U, Uldall P
出版信息
J Clin Microbiol. 1981 Nov;14(5):510-5. doi: 10.1128/jcm.14.5.510-515.1981.
Abstract
An enzyme-linked immunosorbent assay developed for the demonstration of respiratory syncytial (RS) virus immunoglobulin G antibodies was used for the detection of RS virus in specimens of nasopharyngeal secretions (NPS) obtained from children with acute respiratory disease. Samples of NPS were incubated with a fixed amount of standard serum (human serum antibodies to RS virus) before being added to the enzyme-linked immunosorbent assay test plate. A decrease in the optical density value determined for this standard serum was seen with majority of NPS specimens from which RS virus had been isolated in tissue culture. The reliability and the specificity of this inhibiton test were supported by experiments with purified RS virus and by tests with NPS specimens containing other respiratory viruses.
摘要
一种用于检测呼吸道合胞（RS）病毒免疫球蛋白G抗体的酶联免疫吸附测定法，被用于检测从患有急性呼吸道疾病的儿童获取的鼻咽分泌物（NPS）标本中的RS病毒。在将NPS标本添加到酶联免疫吸附测定试验板之前，先将其与固定量的标准血清（抗RS病毒的人血清抗体）进行孵育。在组织培养中已分离出RS病毒的大多数NPS标本中，都观察到该标准血清的光密度值有所下降。用纯化的RS病毒进行的实验以及对含有其他呼吸道病毒的NPS标本进行的检测，均支持了这种抑制试验的可靠性和特异性。
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