Otnaess A B, Halvorsen S
Acta Pathol Microbiol Scand C. 1980 Oct;88(5):247-53. doi: 10.1111/j.1699-0463.1980.tb00102.x.
Milk from 11 Norwegian women was fractionated by ammonium sulphate precipitation and column chromatography. The milk samples inhibited the binding of heat labile E. coli enterotoxin to antibodies coated on microtiter plates in an enzyme-linked immunosorbent assay. Inhibiting activity was not detected when the toxin was measured in an adrenal cell assay. The inhibiting activity was of a non-immunoglobulin nature with an apparent molecular weight of greater than 400 000 in gel filtration experiments.
从11名挪威女性的乳汁中通过硫酸铵沉淀和柱色谱法进行分级分离。在酶联免疫吸附测定中，乳汁样本抑制了热不稳定大肠杆菌肠毒素与包被在微量滴定板上的抗体的结合。在用肾上腺细胞测定法检测毒素时未检测到抑制活性。在凝胶过滤实验中，抑制活性具有非免疫球蛋白性质，表观分子量大于400000。
