Holmgren J, Hanson L A, Carlson B, Lindblad B S, Rahimtoola J
Scand J Immunol. 1976;5(6-7):867-71. doi: 10.1111/j.1365-3083.1976.tb03036.x.
By means of the adrenal cell assay and the rabbit small-bowel loop technique enterotoxin-neutralizing antibodies were demonstrated in all investigated milk samples from severely undernourished Pakistani women but, with a single exception, not in milk from Swedish mothers. The antibodies belonged to the IgA and IgG classes as observed in an enzyme-linked immunosorbent assay, which also revealed secretory component on specific enterotoxin antibodies, showing that the IgA antibodies were primarily of the secretory type. The epidemiological stituation and experimental data strongly indicate that Escherichia coli enterotoxin rather than immunologically cross-reactive Vibrio cholerae toxin in most cases constituted the antigenic stimulus for the milk antibodies. The presence in the milk of the Pakistani mothers of secretory IgA antibodies against an important diarrheogenic agent may have practical importance for protection of the breast-fed baby and probably also illustrates the frequent intestinal exposure of these women to enterotoxinogenic bacteria. Since enterotoxin antigen is presumed not to enter deeper tissues, the demonstration of secretory antibodies in milk agrees with a proposed homing of intestinally triggered lymphocytes to the mammary gland.
通过肾上腺细胞测定法和兔小肠袢技术，在所有来自严重营养不良的巴基斯坦妇女的检测乳样中都证实了存在肠毒素中和抗体，但瑞典母亲的乳汁中，除了一份样本外，均未检测到。在酶联免疫吸附测定中观察到，这些抗体属于IgA和IgG类别，该测定还显示特定肠毒素抗体上存在分泌成分，表明IgA抗体主要是分泌型的。流行病学情况和实验数据有力地表明，在大多数情况下，大肠杆菌肠毒素而非免疫交叉反应性霍乱弧菌毒素构成了乳汁抗体的抗原刺激物。巴基斯坦母亲乳汁中存在针对一种重要致泻因子的分泌型IgA抗体，可能对保护母乳喂养的婴儿具有实际意义，也可能表明这些妇女经常肠道接触产肠毒素细菌。由于肠毒素抗原据推测不会进入更深层组织，乳汁中分泌抗体的存在与肠道触发的淋巴细胞向乳腺归巢的假说相符。
