用于检测嗜肺军团菌抗体的固相免疫荧光测定法的评估
Evaluation of a solid-phase immunofluorescence assay for detection of antibodies to Legionella pneumophila.
作者信息
Thompson T A, Wilkinson H W
出版信息
J Clin Microbiol. 1982 Jul;16(1):202-4. doi: 10.1128/jcm.16.1.202-204.1982.
Abstract
A semiautomated solid-phase immunofluorescence technique (FIAX) was compared with the standard indirect immunofluorescence assay (IFA) for the determination of antibody levels to Legionella pneumophila serogroup 1 in paired human serum samples. The FIAX method was in agreement with the IFA test for 91.8% of the serum pairs but gave evidence of a recent Legionella infection significantly fewer times than did the IFA. These results suggest that the FIAX technique may eventually be a useful alternative test for measuring Legionella antibodies. However, further study will be required to determine its efficacy in providing a serodiagnosis of legionellosis.
摘要
采用半自动固相免疫荧光技术（FIAX）与标准间接免疫荧光测定法（IFA）对配对的人体血清样本中嗜肺军团菌血清1型抗体水平进行了比较。FIAX方法与IFA检测结果对91.8%的血清对一致，但显示近期军团菌感染的证据次数明显少于IFA。这些结果表明，FIAX技术最终可能成为检测军团菌抗体的一种有用的替代检测方法。然而，需要进一步研究以确定其在军团菌病血清诊断中的功效。
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