通过气液色谱-质谱联用技术检测哺乳动物组织中的胞壁酸。
Muramic acid detection in mammalian tissues by gas-liquid chromatography-mass spectrometry.
作者信息
Fox A, Schwab J H, Cochran T
出版信息
Infect Immun. 1980 Aug;29(2):526-31. doi: 10.1128/iai.29.2.526-531.1980.
Abstract
Muramic acid is a component of the peptidoglycan moiety of cell walls of all bacteria and blue-green algae and is not found elsewhere in nature. A gas-liquid chromatography-mass spectrometry assay for muramic acid in tissue is described. The application of the method to the detection of muramic acid in tissues of rats with streptococcal cell wall-induced polyarthritis is demonstrated. Because the method has the potential to measure total bacterial biomass in tissue, it should prove to be an important assay in elucidating the etiological role of bacterial debris in chronic inflammatory diseases in humans.
摘要
胞壁酸是所有细菌和蓝藻细胞壁肽聚糖部分的组成成分，在自然界其他地方不存在。本文描述了一种用于检测组织中胞壁酸的气液色谱 - 质谱分析法。该方法在检测链球菌细胞壁诱导的大鼠多关节炎组织中胞壁酸的应用得到了证实。由于该方法有潜力测量组织中的总细菌生物量，在阐明细菌碎片在人类慢性炎症性疾病中的病因学作用方面，它应被证明是一种重要的分析方法。
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A simple method for the quantitative determination of muramic acid.一种定量测定胞壁酸的简单方法。
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