体内人感染性滑液中微量胞壁酸的绝对鉴定以及无菌性滑液中不存在胞壁酸。
Absolute identification of muramic acid, at trace levels, in human septic synovial fluids in vivo and absence in aseptic fluids.
作者信息
Fox A, Fox K, Christensson B, Harrelson D, Krahmer M
机构信息
Department of Microbiology and Immunology, School of Medicine, University of South Carolina, Columbia, USA.
出版信息
Infect Immun. 1996 Sep;64(9):3911-5. doi: 10.1128/iai.64.9.3911-3915.1996.
Abstract
This is the first report of a study employing the state-of-the-art technique of gas chromatography-tandem mass spectrometry for absolute identification of muramic acid (a marker for peptidoglycan) at trace levels in a human or animal body fluid or tissue. Daughter mass spectra of synovial fluid muramic acid peaks (> or = 30 ng/ml) were identical to those of pure muramic acid. Absolute chemical identification at this level represents a 1,000-fold increase in sensitivity over previous gas chromatography-mass spectrometry identifications. Muramic acid was positively identified in synovial fluids during infection and was eliminated over time but was absent from aseptic fluids.
摘要
这是关于一项研究的首次报告，该研究采用了最先进的气相色谱 - 串联质谱技术，用于在人体或动物体液或组织中痕量水平下绝对鉴定胞壁酸（肽聚糖的一种标志物）。滑液中胞壁酸峰（≥30 ng/ml）的子质谱与纯胞壁酸的子质谱相同。在这个水平上的绝对化学鉴定比以前的气相色谱 - 质谱鉴定的灵敏度提高了1000倍。在感染期间滑液中胞壁酸被明确鉴定出来，并且随着时间推移而消失，但无菌液体中不存在胞壁酸。
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