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Simple filter paper procedure for estimation of glucose uptake via group translocation by whole-cell suspensions of bacteria.通过细菌全细胞悬浮液的基团转位来估计葡萄糖摄取的简单滤纸法。
Appl Environ Microbiol. 1981 Mar;41(3):837-9. doi: 10.1128/aem.41.3.837-839.1981.
2
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Effects of Glucose Concentrations on Cadmium, Copper, Mercury, and Zinc Toxicity to a Klebsiella sp.葡萄糖浓度对 Klebsiella sp. 对镉、铜、汞和锌毒性的影响
Appl Environ Microbiol. 1988 Jul;54(7):1689-93. doi: 10.1128/aem.54.7.1689-1693.1988.
2
Effect of human saliva on the fluoride sensitivity of glucose uptake by Streptococcus mutans.人唾液对变形链球菌摄取葡萄糖的氟敏感性的影响。
Infect Immun. 1981 Dec;34(3):871-9. doi: 10.1128/iai.34.3.871-879.1981.
3
Glucose uptake by Streptococcus mutans, Streptococcus mitis, and Actinomyces viscosus in the presence of human saliva.在人唾液存在的情况下，变形链球菌、缓症链球菌和黏性放线菌对葡萄糖的摄取。
Infect Immun. 1982 Dec;38(3):1060-7. doi: 10.1128/iai.38.3.1060-1067.1982.
4
Transport and incorporation of N-acetyl-D-glucosamine in Bacillus subtilis.N-乙酰-D-葡萄糖胺在枯草芽孢杆菌中的转运与掺入
J Bacteriol. 1982 Apr;150(1):8-15. doi: 10.1128/jb.150.1.8-15.1982.
5
Promotion of Streptococcus mutans glucose transport by human whole saliva and parotid fluid.人全唾液和腮腺液对变形链球菌葡萄糖转运的促进作用。
Infect Immun. 1985 Apr;48(1):7-13. doi: 10.1128/iai.48.1.7-13.1985.
6
Effect of endogenous phosphoenolpyruvate potential on fluoride inhibition of glucose uptake by Streptococcus mutans.内源性磷酸烯醇丙酮酸电位对变形链球菌氟抑制葡萄糖摄取的影响。
Infect Immun. 1986 Jan;51(1):119-24. doi: 10.1128/iai.51.1.119-124.1986.
7
Role of the cell membrane in pH-dependent fluoride inhibition of glucose uptake by Streptococcus mutans.细胞膜在pH依赖性氟化物抑制变形链球菌摄取葡萄糖中的作用。
Antimicrob Agents Chemother. 1986 Jan;29(1):58-61. doi: 10.1128/AAC.29.1.58.

本文引用的文献

1
Role of gal repressor depletion in lambda-dg transduction escape synthesis.半乳糖阻遏物缺失在λ-dg转导逃逸合成中的作用
J Mol Biol. 1970 Jan 28;47(2):121-35. doi: 10.1016/0022-2836(70)90334-7.
2
Effect of dipicolinic acid on the ultraviolet radiation resistance of Bacillus cereus spores.吡啶二羧酸对蜡样芽孢杆菌芽孢抗紫外线辐射能力的影响。
Photochem Photobiol. 1973 Mar;17(3):145-53. doi: 10.1111/j.1751-1097.1973.tb06344.x.
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Phosphoenolpyruvate-dependent sucrose phosphotransferase activity in Streptococcus mutans NCTC 10449.变形链球菌NCTC 10449中磷酸烯醇丙酮酸依赖性蔗糖磷酸转移酶活性
Infect Immun. 1979 Jun;24(3):821-8. doi: 10.1128/iai.24.3.821-828.1979.
4
Direct methods for measuring metabolite transport and distribution in mitochondria.测量线粒体中代谢物转运和分布的直接方法。
Methods Enzymol. 1979;56:279-301. doi: 10.1016/0076-6879(79)56029-7.
5
Distribution of the phosphoenolpyruvate:glucose phosphotransferase system in fermentative bacteria.磷酸烯醇丙酮酸：葡萄糖磷酸转移酶系统在发酵细菌中的分布
J Bacteriol. 1979 Jul;139(1):93-7. doi: 10.1128/jb.139.1.93-97.1979.
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Transport of iron into bacterial cells.
Methods Enzymol. 1979;56:388-94. doi: 10.1016/0076-6879(79)56036-4.
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Glucose transport in Streptococcus agalactiae and its inhibition by lactoperoxidase-thiocyanate-hydrogen peroxide.无乳链球菌中的葡萄糖转运及其受乳过氧化物酶-硫氰酸盐-过氧化氢的抑制作用
J Bacteriol. 1977 Nov;132(2):541-8. doi: 10.1128/jb.132.2.541-548.1977.
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Glycogen synthesis pathway in Streptococcus mutans strain NCTC 10449S and its glycogen synthesis-defective mutant 805.
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通过细菌全细胞悬浮液的基团转位来估计葡萄糖摄取的简单滤纸法。

Simple filter paper procedure for estimation of glucose uptake via group translocation by whole-cell suspensions of bacteria.

作者信息

Germaine G R, Tellefson L M

出版信息

Appl Environ Microbiol. 1981 Mar;41(3):837-9. doi: 10.1128/aem.41.3.837-839.1981.

DOI:10.1128/aem.41.3.837-839.1981
PMID:7013712
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC243785/
Abstract

A simple and rapid filter paper technique is described for processing samples from glucose uptake studies with whole cells of gram-positive or gram-negative bacteria that transport glucose via group translocation. The procedure yields results equivalent to those obtained with a conventional membrane filtration method and requires no special filtration equipment or source of vacuum.

摘要

本文描述了一种简单快速的滤纸技术，用于处理来自革兰氏阳性或革兰氏阴性细菌全细胞葡萄糖摄取研究的样本，这些细菌通过基团转位转运葡萄糖。该方法产生的结果与传统膜过滤法相当，且不需要特殊的过滤设备或真空源。