无乳链球菌中的葡萄糖转运及其受乳过氧化物酶-硫氰酸盐-过氧化氢的抑制作用
Glucose transport in Streptococcus agalactiae and its inhibition by lactoperoxidase-thiocyanate-hydrogen peroxide.
作者信息
Mickelson M N
出版信息
J Bacteriol. 1977 Nov;132(2):541-8. doi: 10.1128/jb.132.2.541-548.1977.
Abstract
Transport of 2-deoxyglucose or glucose in Streptococcus agalactiae was strongly inhibited if the cells were first exposed to a combination of lactoperoxidase-thiocyanate-hydrogen peroxide (LP-complex). The inhibition was completely reversible with dithiothreitol. N-ethylmaleimide and p-chloromercuribenzoate inhibited sugar transport, and the inhibition was also reversible with dithiothreitol. Sodium fluoride also inhibited sugar transport. Glucolysis was completely inhibited, and dithiothreitol completely reversed the inhibition. Phosphoenolpyruvate-dependent phosphotransferase activity in S. agalactiae was not strongly inhibited by the LP-complex. Interference of the entry of glucose into cells of S. agalactiae by the LP-complex could well account for its growth inhibitory properties with this organism. The inhibition of glucose transport by the LP-complex and its reversibility with dithiothreitol suggest the modification of functional sulfhydryl groups in the cell membrane as a cause of transport inhibition.
摘要
如果无乳链球菌细胞首先暴露于乳过氧化物酶 - 硫氰酸盐 - 过氧化氢（LP复合物）的组合中，2 - 脱氧葡萄糖或葡萄糖的转运会受到强烈抑制。用二硫苏糖醇可使这种抑制作用完全逆转。N - 乙基马来酰亚胺和对氯汞苯甲酸抑制糖类转运，且二硫苏糖醇也可逆转这种抑制作用。氟化钠也抑制糖类转运。糖酵解被完全抑制，而二硫苏糖醇可完全逆转这种抑制。无乳链球菌中磷酸烯醇丙酮酸依赖性磷酸转移酶活性未受到LP复合物的强烈抑制。LP复合物对葡萄糖进入无乳链球菌细胞的干扰很可能解释了其对该生物体的生长抑制特性。LP复合物对葡萄糖转运的抑制及其用二硫苏糖醇的可逆性表明细胞膜中功能性巯基的修饰是转运抑制的原因。
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