Săsărman A, Massie B, Zollinger M, Gagne-Tellier H, Shareck F, Garzon S, Morisset R
J Gen Microbiol. 1980 Aug;119(2):475-83. doi: 10.1099/00221287-119-2-475.
Two Escherichia coli strains isolated from urinary tract infections were resistant to streptomycin, kanamycin, neomycin, tetracycline and sulphonamides. The strains also produced colicins B and M. The resistance to streptomycin, kanamycin and neomycin and the ability to produce colicins B and M could be transferred to an E. coli K12 recipient. Resistance and colicinogeny markers were transferred together by conjugation, and did not segregate even after interrupted mating or phage P1-mediated transduction. Hence, the drug-resistance and colicinogeny markers were carried by the same plasmid, designated as R.ColBM plasmid. The two R.ColBM plasmids were Fi+ and produced an F-like pilus. They belonged to the IncFIII incompatibility group, being thus the first R plasmids identified in this group. The two plasmids were isolated and their molecular sizes were determined by electrophoresis in agarose gels and by contour length measurements. Both methods showed that both plasmids were about 52 megadaltons.
从尿路感染中分离出的两株大肠杆菌对链霉素、卡那霉素、新霉素、四环素和磺胺类药物具有抗性。这些菌株还产生大肠杆菌素B和M。对链霉素、卡那霉素和新霉素的抗性以及产生大肠杆菌素B和M的能力可转移至大肠杆菌K12受体菌。抗性和大肠杆菌素ogeny标记通过接合共同转移，即使在中断交配或噬菌体P1介导的转导后也不分离。因此，耐药性和大肠杆菌素ogeny标记由同一质粒携带，命名为R.ColBM质粒。这两个R.ColBM质粒是Fi+，并产生F样菌毛。它们属于IncFIII不相容群，因此是该群中鉴定出的首个R质粒。分离出这两个质粒，并通过琼脂糖凝胶电泳和轮廓长度测量确定其分子大小。两种方法均表明这两个质粒约为52兆道尔顿。
