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藤黄微球菌和大肠杆菌提取物对烷基化DNA中7-甲基鸟嘌呤残基的释放作用

Release of 7-methylguanine residues from alkylated DNA by extracts of Micrococcus luteus and Escherichia coli.

作者信息

Laval J, Pierre J, Laval F

出版信息

Proc Natl Acad Sci U S A. 1981 Feb;78(2):852-5. doi: 10.1073/pnas.78.2.852.

DOI:10.1073/pnas.78.2.852
PMID:7015333
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC319901/
Abstract

Cell extracts from Micrococcus luteus release both free 3-methyladenine and free 7-methylguanine from alkylated DNA. The glycosylase activity responsible for the liberation of 7-methylguanine is not 3-methyladenine-DNA glycosylase, which, when purified, does not liberate it. Furthermore, the heat inactivation rates of the two enzymatic activities are different. The release of 7-methylguanine by chemical depurination of ethanol-soluble oligonucleotides has been ruled out. A similar activity releasing 7-methylguanine is also found in Escherichia coli.

摘要

藤黄微球菌的细胞提取物能从烷基化DNA中释放出游离的3-甲基腺嘌呤和游离的7-甲基鸟嘌呤。负责释放7-甲基鸟嘌呤的糖基化酶活性并非3-甲基腺嘌呤-DNA糖基化酶，后者纯化后不会释放7-甲基鸟嘌呤。此外，这两种酶活性的热失活速率不同。通过乙醇可溶性寡核苷酸的化学脱嘌呤作用释放7-甲基鸟嘌呤的可能性已被排除。在大肠杆菌中也发现了一种释放7-甲基鸟嘌呤的类似活性。

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Release of 7-methylguanine residues from alkylated DNA by extracts of Micrococcus luteus and Escherichia coli.藤黄微球菌和大肠杆菌提取物对烷基化DNA中7-甲基鸟嘌呤残基的释放作用
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本文引用的文献

1
FURTHER STUDIES ON THE ALKYLATION OF NUCLEIC ACIDS AND THEIR CONSTITUENT NUCLEOTIDES.核酸及其组成核苷酸烷基化的进一步研究
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Differential destruction of the transforming activity of damaged deoxyribonucleic acid by a bacterial enzyme.一种细菌酶对受损脱氧核糖核酸转化活性的差异性破坏。
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Decreased excision of O6-methylguanine and N7-methylguanine during the S phase in 10T1/2 cells.10T1/2细胞在S期O6-甲基鸟嘌呤和N7-甲基鸟嘌呤的切除减少。
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Enzymatic release of 7-methylguanine from methylated DNA by rodent liver extracts.啮齿动物肝脏提取物对甲基化DNA中7-甲基鸟嘌呤的酶促释放。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):861-5. doi: 10.1073/pnas.78.2.861.
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Human lymphoblasts contain DNA glycosylase activity excising N-3 and N-7 methyl and ethyl purines but not O6-alkylguanines or 1-alkyladenines.人类淋巴母细胞含有DNA糖基化酶活性，可切除N-3和N-7甲基及乙基嘌呤，但不能切除O6-烷基鸟嘌呤或1-烷基腺嘌呤。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):856-60. doi: 10.1073/pnas.78.2.856.
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Micrococcus luteus endonucleases for apurinic/apyrimidinic sites in deoxyribonucleic acid. 2. Further studies on the substrate specificity and mechanism of action.用于脱氧核糖核酸中无嘌呤/无嘧啶位点的藤黄微球菌核酸内切酶。2. 关于底物特异性和作用机制的进一步研究。
Biochemistry. 1980 Oct 28;19(22):5024-9. doi: 10.1021/bi00563a014.
7
Micrococcus luteus endonucleases for apurinic/apyrimidinic sites in deoxyribonucleic acid. 1. Purification and general properties.用于脱氧核糖核酸中脱嘌呤/脱嘧啶位点的藤黄微球菌核酸内切酶。1. 纯化及一般性质。
Biochemistry. 1980 Oct 28;19(22):5018-24. doi: 10.1021/bi00563a013.
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Specific excision of methylation products from DNA of Escherichia coli treated with N-methyl-N'-nitro-N-nitrosoguanidine.从用N-甲基-N'-硝基-N-亚硝基胍处理的大肠杆菌DNA中特异性切除甲基化产物
Chem Biol Interact. 1970 Aug;2(2):154-7. doi: 10.1016/0009-2797(70)90047-5.
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Group separation of ribonucleotides, ribonucleosides, and purine and pyrimidine bases on polyacrylamide gel columns.核糖核苷酸、核糖核苷以及嘌呤和嘧啶碱基在聚丙烯酰胺凝胶柱上的分组分离。
Anal Biochem. 1974 Apr;58(2):638-41. doi: 10.1016/0003-2697(74)90234-6.
10
The enzymatic release of O6-methylguanine and 3-methyladenine from DNA reacted with the carcinogen N-methyl-N-nitrosourea.与致癌物N-甲基-N-亚硝基脲反应的DNA中O6-甲基鸟嘌呤和3-甲基腺嘌呤的酶促释放。
Proc Natl Acad Sci U S A. 1974 May;71(5):2022-6. doi: 10.1073/pnas.71.5.2022.