藤黄微球菌和大肠杆菌提取物对烷基化DNA中7-甲基鸟嘌呤残基的释放作用
Release of 7-methylguanine residues from alkylated DNA by extracts of Micrococcus luteus and Escherichia coli.
作者信息
Laval J, Pierre J, Laval F
出版信息
Proc Natl Acad Sci U S A. 1981 Feb;78(2):852-5. doi: 10.1073/pnas.78.2.852.
Abstract
Cell extracts from Micrococcus luteus release both free 3-methyladenine and free 7-methylguanine from alkylated DNA. The glycosylase activity responsible for the liberation of 7-methylguanine is not 3-methyladenine-DNA glycosylase, which, when purified, does not liberate it. Furthermore, the heat inactivation rates of the two enzymatic activities are different. The release of 7-methylguanine by chemical depurination of ethanol-soluble oligonucleotides has been ruled out. A similar activity releasing 7-methylguanine is also found in Escherichia coli.
摘要
藤黄微球菌的细胞提取物能从烷基化DNA中释放出游离的3-甲基腺嘌呤和游离的7-甲基鸟嘌呤。负责释放7-甲基鸟嘌呤的糖基化酶活性并非3-甲基腺嘌呤-DNA糖基化酶，后者纯化后不会释放7-甲基鸟嘌呤。此外，这两种酶活性的热失活速率不同。通过乙醇可溶性寡核苷酸的化学脱嘌呤作用释放7-甲基鸟嘌呤的可能性已被排除。在大肠杆菌中也发现了一种释放7-甲基鸟嘌呤的类似活性。
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本文引用的文献
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FURTHER STUDIES ON THE ALKYLATION OF NUCLEIC ACIDS AND THEIR CONSTITUENT NUCLEOTIDES.核酸及其组成核苷酸烷基化的进一步研究
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Decreased excision of O6-methylguanine and N7-methylguanine during the S phase in 10T1/2 cells.10T1/2细胞在S期O6-甲基鸟嘌呤和N7-甲基鸟嘌呤的切除减少。
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Micrococcus luteus endonucleases for apurinic/apyrimidinic sites in deoxyribonucleic acid. 1. Purification and general properties.用于脱氧核糖核酸中脱嘌呤/脱嘧啶位点的藤黄微球菌核酸内切酶。1. 纯化及一般性质。
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