聚（dG-dC）·聚（dG-dC）中的7-甲基鸟嘌呤促进Z-DNA的形成。
7-Methylguanine in poly(dG-dC).poly(dG-dC) facilitates z-DNA formation.
作者信息
Möller A, Nordheim A, Nichols S R, Rich A
出版信息
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4777-81. doi: 10.1073/pnas.78.8.4777.
Abstract
Poly(dG-dC).poly(dG-dC) was methylated at the N-7 position of guanine through the use of dimethyl sulfate. The conversion of poly(dG-dC).poly(dG-dC) from B-DNA to the Z-DNA form was followed by measuring both the circular dichroic spectra and changes in the absorbance ratio A295/A260. Increasing methylation steadily decreases the amount of NaCl or MgCl2 that is required to convert the polymer from B-DNA to Z-DNA. At 100% methylation of the guanine residues, the modified polymer is fully converted to Z-DNA in a physiological salt solution. Kinetic experiments show that methylation markedly accelerates the speed of the conversion from B-DNA to Z-DNA in the presence of added salt. These effects may be partly due to the positive charge on guanine that accompanies N-7 methylation.
摘要
通过使用硫酸二甲酯，聚（dG-dC）·聚（dG-dC）在鸟嘌呤的N-7位被甲基化。通过测量圆二色光谱和吸光度比值A295/A260的变化来跟踪聚（dG-dC）·聚（dG-dC）从B-DNA向Z-DNA形式的转变。甲基化程度的增加会稳步减少将聚合物从B-DNA转变为Z-DNA所需的NaCl或MgCl2的量。在鸟嘌呤残基100%甲基化时，修饰后的聚合物在生理盐溶液中完全转变为Z-DNA。动力学实验表明，在添加盐的情况下，甲基化显著加速了从B-DNA向Z-DNA的转变速度。这些效应可能部分归因于N-7甲基化伴随的鸟嘌呤上的正电荷。
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Release of 7-methylguanine residues from alkylated DNA by extracts of Micrococcus luteus and Escherichia coli.藤黄微球菌和大肠杆菌提取物对烷基化DNA中7-甲基鸟嘌呤残基的释放作用
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The tetramer d(CpGpCpG) crystallizes as a left-handed double helix.四聚体d（CpGpCpG）结晶为左手双螺旋结构。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4016-20. doi: 10.1073/pnas.77.7.4016.
9
Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).甲基化对合成多核苷酸的影响：聚（dG-m5dC）·聚（dG-m5dC）中的B-Z转变
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