部分肝切除对大鼠肝脏提取物从烷基化DNA中去除O6-甲基鸟嘌呤的影响。
Effect of partial hepatectomy on removal of O6-methylguanine from alkylated DNA by rat liver extracts.
作者信息
Pegg A E, Perry W, Bennett R A
出版信息
Biochem J. 1981 Jul 1;197(1):195-201. doi: 10.1042/bj1970195.
Abstract
- The activity of an enzyme catalysing the loss of O6-methylguanine from methylated DNA was increasing during liver regeneration after partial hepatectomy. Activity was increased 3-fold by 24h and was maximal (6-fold increase) over the period 48-72h after operation. 2. This activity could also be induced by chronic treatment with dimethylnitrosamine, but the maximal response amounted to a 2-3-fold change (with the greater effect in male rats) after 4-6 weeks of exposure to daily doses of 2 mg of dimethylnitrosamine/kg. 3. Neither partial hepatectomy nor treatment with dimethylnitrosamine increased the activities of two other enzymes repairing alkylated DNA, DNA (7-methylguanine-)glycosylase and DNA (3-methyladenine-)glycosylase. 4. These results therefore indicate that there is a selective induction of the O6-methylguanine removal system during hepatocyte proliferation. Since this product is known to lead to mutations and its persistence in DNA throughout cell replication has been implicated in tumour initiation, this induction may play a role in resistance to carcinogenesis by alkylating agents.
摘要
- 在部分肝切除术后肝脏再生过程中，催化甲基化DNA上O6-甲基鸟嘌呤去除的酶的活性增强。术后24小时活性增加了3倍，在术后48 - 72小时达到最大值（增加了6倍）。2. 用二甲基亚硝胺长期处理也可诱导这种活性，但在每天给予2毫克/千克二甲基亚硝胺，连续暴露4 - 6周后，最大反应为2 - 3倍变化（雄性大鼠的效应更大）。3. 部分肝切除或用二甲基亚硝胺处理均未增加另外两种修复烷基化DNA的酶，即DNA（7-甲基鸟嘌呤）糖基化酶和DNA（3-甲基腺嘌呤）糖基化酶的活性。4. 因此，这些结果表明在肝细胞增殖过程中存在O6-甲基鸟嘌呤去除系统的选择性诱导。由于已知该产物会导致突变，并且其在整个细胞复制过程中在DNA中的持续存在与肿瘤起始有关，这种诱导可能在对烷基化剂致癌作用的抗性中发挥作用。
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本文引用的文献
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Adaptive response to alkylating agents involves alteration in situ of O6-methylguanine residues in DNA.对烷化剂的适应性反应涉及DNA中O6-甲基鸟嘌呤残基的原位改变。
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Quantitation of the adaptive response to alkylating agents.烷化剂适应性反应的定量分析。
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Removal of O6-methylguanine from DNA of normal and xeroderma pigmentosum-derived lymphoblastoid lines.从正常及着色性干皮病来源的淋巴母细胞系的DNA中去除O6-甲基鸟嘌呤。
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Sequential stimulation of DNA repair and DNA replication in normal human cells.正常人类细胞中DNA修复和DNA复制的顺序刺激。
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Decreased excision of O6-methylguanine and N7-methylguanine during the S phase in 10T1/2 cells.10T1/2细胞在S期O6-甲基鸟嘌呤和N7-甲基鸟嘌呤的切除减少。
Biochem Biophys Res Commun. 1980 Feb 12;92(3):787-94. doi: 10.1016/0006-291x(80)90772-x.
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Enhanced excision of O6-alkylguanine in rat liver by pretreatment with acetylaminofluorene.通过乙酰氨基芴预处理增强大鼠肝脏中O6-烷基鸟嘌呤的切除。
Nature. 1980 Mar 27;284(5754):378. doi: 10.1038/284378a0.
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Enzymatic release of 7-methylguanine from methylated DNA by rodent liver extracts.啮齿动物肝脏提取物对甲基化DNA中7-甲基鸟嘌呤的酶促释放。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):861-5. doi: 10.1073/pnas.78.2.861.
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Changes in O6-methylguanine disappearance from rat liver DNA during chronic dimethylnitrosamine administration. A possible similarity between the system removing O6-methylguanine from DNA in rat liver and in Escherichia coli adapted to N-methyl-N'-nitro-N-nitrosoguanidine.慢性给予二甲基亚硝胺期间大鼠肝脏DNA中O6-甲基鸟嘌呤消失情况的变化。大鼠肝脏和适应N-甲基-N'-硝基-N-亚硝基胍的大肠杆菌中从DNA去除O6-甲基鸟嘌呤的系统之间可能存在的相似性。
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