用于检测沙粒病毒的酶联免疫吸附测定法。
Enzyme-linked immunosorbent assay for detection of arenaviruses.
作者信息
Ivanov A P, Bashkirtsev V N, Tkachenko E A
出版信息
Arch Virol. 1981;67(1):71-4. doi: 10.1007/BF01314603.
PMID:7016080
Abstract
Conditions for ELISA by using IgG linked with horseradish peroxidase have been developed for the detection of 6 arenaviruses (Machupo, Junin, Tacaribe, Amapari, LCM and Tamiami). This method allows the detection of arenavirus antigens in various materials; organs and blood in affected animals and infected cell culture fluids. It shows higher sensitivity and allows an earlier detection of virus-specific antigens as compared with the CF test.
摘要
已开发出利用与辣根过氧化物酶相连的IgG进行酶联免疫吸附测定（ELISA）的条件，用于检测6种沙粒病毒（马丘波病毒、胡宁病毒、塔卡里贝病毒、阿马帕里病毒、淋巴细胞脉络丛脑膜炎病毒和塔米亚米病毒）。该方法可检测各种材料中的沙粒病毒抗原；受感染动物的器官和血液以及感染的细胞培养液。与补体结合试验相比，它显示出更高的灵敏度，并能更早地检测到病毒特异性抗原。
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