用于鉴定肠杆菌科细菌的自动微生物系统的实验室评估。
Laboratory evaluation of the AutoMicrobic system for identification of Enterobacteriaceae.
作者信息
Freeman J W, Rowland R W, Overman S B, Goodman N L
出版信息
J Clin Microbiol. 1981 May;13(5):895-8. doi: 10.1128/jcm.13.5.895-898.1981.
The Enterobacteriaceae Biochemical Card used with the AutoMicrobic system (Vitek Systems, Inc., Hazelwood, Mo.) was compared with the API 20E system (Analytab Products, Plainview, N.Y.) for identifying members of the family Enterobacteriaceae. A total of 1,401 clinical isolates representing 18 species were identified by the AutoMicrobic and API 20E systems over a 5-month period. Discrepancies between these systems were resolved by conventional methods. Overall, 98.3% of the isolates were identified correctly by the AutoMicrobic system in 8 h, with 94.2% having an AutoMicrobic system confidence level greater than or equal to 90%; 92.9% of the isolates were identified correctly by the 20E system after 24 h of incubation. Discrepancies between the two systems occurred in 3.1% of the isolates. Of these, 40.9 and 59.1% proved to be correct by the AutoMicrobic and API 20E systems, respectively.
将用于自动微生物系统（Vitek系统公司，密苏里州黑兹尔伍德）的肠杆菌科生化鉴定卡与API 20E系统（Analytab产品公司，纽约州普莱恩维尤）进行比较，以鉴定肠杆菌科成员。在5个月的时间里，自动微生物系统和API 20E系统共鉴定了代表18个菌种的1401株临床分离株。这些系统之间的差异通过传统方法得以解决。总体而言，自动微生物系统在8小时内正确鉴定了98.3%的分离株，其中94.2%的自动微生物系统置信水平大于或等于90%；20E系统在培养24小时后正确鉴定了92.9%的分离株。两个系统之间的差异出现在3.1%的分离株中。其中，自动微生物系统和API 20E系统分别证明40.9%和59.1%是正确的。
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