March R E, Reeback J S, Holborow E J, Coombs R R
J Immunol Methods. 1981;42(2):137-46. doi: 10.1016/0022-1759(81)90143-5.
A simple and inexpensive microtitre plate test (the mixed reverse (solid-phase) passive antiglobulin haemadherence test, or MRSPAH) has been developed for the measurement of antiglobulins (RFS) of different classes. Results obtained for IgM RF by this test have been compared with results of latex and Rose-Waaler (DAT) tests on rheumatoid arthritis (RA) sera. Levels of RFs of IgM, IgG and IgA classes have been measured by MRSPAH using rabbit IgG as antigen in RAs and normal people. 94% of RA sera tested were above the upper limit of normal for IgM and/or IgA RF. There was a considerable overlap between IgG RF levels in RAs and normals, although the means of the two groups were significantly different.
已开发出一种简单且成本低廉的微量滴定板检测方法（混合反向（固相）被动抗球蛋白血细胞黏附试验，即MRSPAH），用于检测不同类别的抗球蛋白（类风湿因子，RFS）。通过该检测方法获得的IgM类风湿因子检测结果，已与类风湿性关节炎（RA）血清的乳胶和玫瑰-瓦勒（直接抗球蛋白试验，DAT）检测结果进行了比较。使用兔IgG作为抗原，通过MRSPAH测定了RA患者和正常人中IgM、IgG和IgA类类风湿因子的水平。94%检测的RA血清中IgM和/或IgA类风湿因子高于正常上限。尽管两组的平均值存在显著差异，但RA患者和正常人的IgG类风湿因子水平有相当大的重叠。
