Spira W M, Sack R B, Froehlich J L
Infect Immun. 1981 May;32(2):739-47. doi: 10.1128/iai.32.2.739-747.1981.
We developed an adult rabbit model for enteric infection by Vibrio cholerae and enterotoxigenic Escherichia coli. The cecum of each animal was first ligated to prevent it from retaining fluid secreted by the small intestine. A temporary reversible obstruction (a slip knot tie) of the small bowel was introduced at the time of challenge and removed 2 h later. With this modification, we were able to elicit a massive and usually fatal cholera-like diarrhea in adult (3.5- to 6-lb [1.6- to 2.7-kb]) animals challenged with V. cholerae. Animals challenged with enterotoxigenic E. coli also developed diarrhea which was severe and watery but less explosive and less rapidly fatal than that produced by V. cholerae. The susceptibility of animals in this model to infection by V. cholerae was similar to the susceptibility of infant rabbits challenged intraintestinally. The death rate was almost 25% when 10(3) Vibrio cells were given and 90% or more when the dose was greater than or equal to 10(6) cells per animal. We designated this procedure the RITARD (for removable intestinal tie-adult rabbit diarrhea) model.
我们开发了一种成年兔模型，用于研究霍乱弧菌和产肠毒素大肠杆菌引起的肠道感染。首先结扎每只动物的盲肠，以防止其保留小肠分泌的液体。在接种时对小肠进行临时可逆性梗阻（打活结），2小时后解除。通过这种改良，我们能够在成年（体重3.5至6磅[1.6至2.7千克]）动物中引发大量且通常致命的霍乱样腹泻，这些动物用霍乱弧菌进行攻击。用产肠毒素大肠杆菌攻击的动物也会出现腹泻，腹泻严重且呈水样，但比霍乱弧菌引起的腹泻发作性较弱，致死速度也较慢。该模型中动物对霍乱弧菌感染的易感性与经肠道接种攻击的幼兔相似。当每只动物给予10³个霍乱弧菌细胞时，死亡率约为25%；当剂量大于或等于每只动物10⁶个细胞时，死亡率达到90%或更高。我们将此程序命名为RITARD（可移除肠结扎 - 成年兔腹泻）模型。
