Wilmer J, Schubert J
Mutat Res. 1981 Apr;88(4):337-42. doi: 10.1016/0165-1218(81)90024-0.
Solutions of nucleic acid bases, nucleosides and a nucleotide, saturated with either N2, N2O or O2, were irradiated and tested for mutagenicity towards Salmonella typhimurium, with and without pre-incubation. Irradiated solutions of the nucleic acid bases were all non-mutagenic. Irradiated solutions of the nucleosides showed mutagenicity in S. typhimurium TA100 (pre-incubation assay). Generally, the mutagenicity followed the order: N2O greater than N2 greater than O2. The results show that the formation of mutagenic radiolytic products is initiated by attack of mainly OH radicals on the 2-deoxy-D-ribose moiety of the nucleosides. With irradiated solutions of the nucleotide, thymidine-5'-monophosphate, no mutagenicity could be detected.
将用N₂、N₂O或O₂饱和的核酸碱基、核苷和一种核苷酸溶液进行辐照，并检测其对鼠伤寒沙门氏菌的诱变性，同时进行预孵育和不进行预孵育的实验。辐照后的核酸碱基溶液均无诱变性。辐照后的核苷溶液在鼠伤寒沙门氏菌TA100中表现出诱变性（预孵育试验）。一般来说，诱变性顺序为：N₂O＞N₂＞O₂。结果表明，诱变辐射分解产物的形成主要是由OH自由基攻击核苷的2-脱氧-D-核糖部分引发的。对于辐照后的核苷酸溶液，即胸苷-5'-单磷酸，未检测到诱变性。
