Doyle M J, Maclean N
J Embryol Exp Morphol. 1978 Aug;46:215-25.
Premetamorphic tadpoles of Xenopus laevis reared in water containing 0.01% propylthiouracil are developmentally retarded and metamorphosis is prevented. When uncrowded, they continue to grow to a giant size. Moderate crowding leads to a slower rate of growth. Thus morphologically premetamorphic tadpoles were produced with lens diameters appropriate to either normal premetamorphic, climactic or post-metamorphic animals. The lens crystallins of such tadpoles have been separated by immunoelectrophoresis and polyacrylamide gel electrophoresis. The crystallin pattern was correlated with lens diameter rather than with body stage. Giant retarded larvae possessed crystallin patterns identical to those or normal adults. The thyroid antagonist propylthiouracil is therefore unable to prevent the lens crystallin transition, the beginning of which is normally temporally correlated with metamorphosis.
在含有0.01%丙基硫氧嘧啶的水中饲养的非洲爪蟾前变态期蝌蚪发育迟缓，变态被阻止。在不拥挤的情况下，它们会继续生长到巨大的体型。适度拥挤会导致生长速度变慢。因此，产生了形态上前变态期的蝌蚪，其晶状体直径与正常前变态期、高峰期或变态后动物的晶状体直径相当。通过免疫电泳和聚丙烯酰胺凝胶电泳分离了这些蝌蚪的晶状体晶状体蛋白。晶状体蛋白模式与晶状体直径相关，而不是与身体阶段相关。巨大的发育迟缓幼虫具有与正常成年动物相同的晶状体蛋白模式。因此，甲状腺拮抗剂丙基硫氧嘧啶无法阻止晶状体晶状体蛋白的转变，而这种转变的开始通常在时间上与变态相关。
