Bowers L D, Wong E T
Clin Chem. 1980 Apr;26(5):555-61.
We have evaluated the Technicon AutoAnalyzer method, the Beckman Creatinine Analyzer II, DuPont aca, Electro-Nucleonics GEMSAEC, and Instrumentation Laboratory Model 916 kinetic methods for determination of serum creatinine. These methods, which involve the Jaffé reaction, all showed a statistically significant proportional error relative to an ion-exchange pretreatment method for creatinine in 92 serum samples; the GEMSAEC method also showed a bias. The positive interference of several acid metabolites was quantitated in each of the methods. The evidence indicates that bilirubin, although associated with a negative creatinine interference, is not the sole interferent. We determined the magnitude of this interference and studied several methods for minimizing it.
我们评估了Technicon自动分析仪法、贝克曼肌酐分析仪II、杜邦aca、电子核子学GEMSAEC以及仪器实验室916型动力学法测定血清肌酐的情况。这些涉及Jaffé反应的方法，相对于一种用于92份血清样本中肌酐的离子交换预处理方法，均显示出具有统计学意义的比例误差；GEMSAEC法还显示出偏差。在每种方法中对几种酸性代谢物的正干扰进行了定量。证据表明，胆红素虽然与肌酐的负干扰有关，但并非唯一的干扰物。我们确定了这种干扰的程度，并研究了几种将其降至最低的方法。
