用于快速鉴定肠杆菌科细菌的MS-2系统的评估
Evaluation of the MS-2 system for rapid identification of Enterobacteriaceae.
作者信息
McCracken A W, Martin W J, McCarthy L R, Schwab D A, Cooper B H, Helgeson N G, Prowant S, Robson J
出版信息
J Clin Microbiol. 1980 Nov;12(5):684-9. doi: 10.1128/jcm.12.5.684-689.1980.
The precision, accuracy, and other performance characteristics of the MS-2 (Abbott Laboratories, Diagnostic Division, Dallas, Tex.) system for the identification of Enterobacteriaceae were evaluated in a collaborative study involving three clinical laboratories. When identifying 150 unknown, coded organisms, the MS-2 system was 97%, 98%, and 93% accurate, respectively, in three laboratories. The system showed an overall accuracy of 94% when compared with conventional manual tube methods in identifying 1,154 clinical isolates of 26 species of Enterobacteriaceae. Discrepancies between automated and conventional methods were chiefly caused by biochemical variants, especially among Enterobacter species. The MS-2 system was rapid and simple to operate and produced printed results of bacterial identification in 5 h. The cost of disposable components compared favorably with commercial, visually read systems for identifying Enterobacteriaceae.
在一项涉及三个临床实验室的协作研究中，对用于鉴定肠杆菌科细菌的MS - 2系统（雅培实验室诊断部，得克萨斯州达拉斯）的精密度、准确度及其他性能特征进行了评估。在鉴定150株未知编码菌株时，MS - 2系统在三个实验室中的准确率分别为97%、98%和93%。在鉴定26种肠杆菌科细菌的1154株临床分离株时，与传统手动试管法相比，该系统的总体准确率为94%。自动化方法与传统方法之间的差异主要由生化变异体引起，尤其是在肠杆菌属细菌中。MS - 2系统操作快速简便，5小时内即可生成细菌鉴定的打印结果。与用于鉴定肠杆菌科细菌的商业目视读取系统相比，一次性组件的成本更具优势。
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