Raul F, Kedinger M, Simon P M, Grenier J F, Haffen K
Biol Neonate. 1981;39(3-4):200-7. doi: 10.1159/000241437.
Suckling rats were bottle fed during 48 h with a basic diet enriched with different mono- or disaccharides. In parallel, explants of intestinal mucosa were cultured in vitro for 48 h in the presence or in the absence of a synthetic glucocorticoid (dexamethasone) and with or without different carbohydrates. In both studies, enzyme activities were assayed on purified brush border membranes. From these combined in vivo and in vitro investigations it appeared that (1) there is a highly specific stimulation of mono- and disaccharides on the corresponding brush border disaccharidases: glucose, fructose, sucrose on sucrase and maltase activities, fructose being generally the most potent activator. Other brush border enzymes were not modified by the dietary carbohydrates. (2) These sugar-mediated effects were obtained only in the presence of glucocorticoids. This hormone alone induced the appearance of a slight sucrase activity and provoked a stimulation of maltase activity. The results show clearly that glucocorticoids are necessary to induce sucrase activity, but that the level of this activity is under the strict dependence of the dietary carbohydrates.
给乳鼠用添加了不同单糖或双糖的基础饮食进行48小时奶瓶喂养。同时，将肠黏膜外植体在有或没有合成糖皮质激素（地塞米松）以及有或没有不同碳水化合物的情况下进行48小时体外培养。在这两项研究中，均在纯化的刷状缘膜上测定酶活性。从这些体内和体外的联合研究中可以看出：（1）单糖和双糖对相应的刷状缘双糖酶有高度特异性的刺激作用：葡萄糖、果糖、蔗糖对蔗糖酶和麦芽糖酶活性有刺激作用，果糖通常是最有效的激活剂。其他刷状缘酶未被饮食中的碳水化合物改变。（2）这些糖介导的效应仅在糖皮质激素存在的情况下才会出现。单独这种激素会诱导轻微的蔗糖酶活性出现，并刺激麦芽糖酶活性。结果清楚地表明，糖皮质激素是诱导蔗糖酶活性所必需的，但该活性水平严格依赖于饮食中的碳水化合物。
