James P S, Smith M W, Butcher G W, Brown D, Lund E K
Biochem Genet. 1986 Apr;24(3-4):169-81. doi: 10.1007/BF00502786.
Assays for sucrase carried out on intestinal sonicates prepared from 18 different strains of mice revealed a threefold variation in specific activity, the values for CBA/Ca mice being significantly less than for any other strain. Further comparison of the CBA/Ca versus the C57BL/6J mouse showed this deficiency, which became established 2-4 weeks after birth, to apply to isomaltase as well as sucrase but not to maltase or trehalase. Backcross experiments indicated that this deficiency in sucrase activity was inherited as a single codominantly expressed genetic factor. The ability of the CBA/Ca mouse to regulate sucrase activity in response to changes in diet was also reduced compared to that of the C57BL/6J mouse. No difference could be detected in the affinity of sucrase for its substrate or in the ability of heat to denature sucrase prepared from CBA/Ca and C57BL/6J mice. It is suggested that part of the regulatory region of the gene coding for sucrase-isomaltase is modified in the CBA/Ca mouse and that this locus should be given the notation Suc-1 for future reference.
对从18种不同品系小鼠制备的肠超声匀浆进行的蔗糖酶测定显示，比活性有三倍的差异，CBA/Ca小鼠的值显著低于任何其他品系。对CBA/Ca小鼠与C57BL/6J小鼠的进一步比较表明，这种在出生后2至4周确立的缺陷适用于异麦芽糖酶和蔗糖酶，但不适用于麦芽糖酶或海藻糖酶。回交实验表明，蔗糖酶活性的这种缺陷作为一个单一的共显性表达遗传因子遗传。与C57BL/6J小鼠相比，CBA/Ca小鼠响应饮食变化调节蔗糖酶活性的能力也降低。在蔗糖酶对其底物的亲和力或热变性CBA/Ca和C57BL/6J小鼠制备的蔗糖酶的能力方面未检测到差异。有人提出，编码蔗糖酶-异麦芽糖酶的基因的部分调控区域在CBA/Ca小鼠中发生了改变，并且该基因座应标记为Suc-1以供将来参考。
