Elango N, Correa J U, Cabib E
J Biol Chem. 1982 Feb 10;257(3):1398-400.
During the conversion of yeast cells to protoplasts, about half of the yeast chitinase was liberated into the medium, an indication that this portion of the enzyme is located in the periplasmic space. At least part of the remaining chitinase appears to be enclosed in vacuoles or in vesicle that co-purify with them, as indicated by a 14-fold enrichment of the enzymatic activity in a vacuole fraction isolated from a protoplast lysate. When protoplasts were incubated in growth medium, part of the chitinase was liberated in growth medium, part of the chitinase was liberated into the medium. It is concluded that yeast chitinase is a secretory enzyme, like invertase and acid phosphatase. The enzyme appears to be stored in vesicles as a prelude to its secretion into the periplasmic space. The possible function of yeast chitinase in cell division is discussed.
在酵母细胞转化为原生质体的过程中，约一半的酵母几丁质酶释放到培养基中，这表明该部分酶位于周质空间。从原生质体裂解物中分离得到的液泡组分中酶活性富集了14倍，这表明至少部分剩余的几丁质酶似乎被包裹在液泡或与其共纯化的囊泡中。当原生质体在生长培养基中孵育时，部分几丁质酶释放到生长培养基中。结论是酵母几丁质酶是一种分泌酶，类似于转化酶和酸性磷酸酶。该酶似乎以囊泡形式储存，作为其分泌到周质空间的前奏。还讨论了酵母几丁质酶在细胞分裂中的可能功能。
