Nishimura H, Sempuku K, Iwashima A
J Bacteriol. 1982 May;150(2):960-2. doi: 10.1128/jb.150.2.960-962.1982.
Thiamine was found to be accumulated in protoplasts of Saccharomyces cerevisiae in the same manner as in intact cells, suggesting that a soluble thiamine-binding protein in periplasm may not be an essential component of the thiamine transport system of S. cerevisiae. It was also found that thiamine pyrophosphate cannot be taken up by yeast protoplasts.
人们发现硫胺素在酿酒酵母原生质体中的积累方式与在完整细胞中相同，这表明周质中一种可溶性硫胺素结合蛋白可能不是酿酒酵母硫胺素转运系统的必需成分。还发现硫胺素焦磷酸不能被酵母原生质体吸收。
