Van Wyk J A, Gerber H M
Onderstepoort J Vet Res. 1978 Mar;45(1):29-37.
When gastro-intestinal ingesta and mucosa containing larvae or adult Haemonchus contortus, Ostertagia circumcincta. Trichostrongylus colubriformis, Strongyloides papillosus, Nematodirus spathiger, Gaigeria pachyscelis, Oesophagostomum columbianum or Chabertia ovina were suspended in 0,75--1,00% agar gel and incubated in physiological saline, a mean of 93,6% of the helminths migrated from the agar-ingesta or agar-mucosa into the physiological saline. The lowest suitable concentration of agar was 0,85--0,90% for one batch of agar and 0,65% for another. Although most of the worms had migrated from the agar gel after 3--4 h of incubation, migration usually continued for longer than 7 h. While low concentrations of formalin partially inhibited migration from the gel, slightly more worms were recovered from agar containing 1% bile than from agar alone.
当含有捻转血矛线虫、环形奥斯特线虫、蛇形毛圆线虫、乳头类圆线虫、粗纹食道口线虫、厚尾盖氏线虫、哥伦比亚食道口线虫或绵羊夏伯特线虫幼虫或成虫的胃肠内容物及黏膜悬浮于0.75% - 1.00%的琼脂凝胶中，并在生理盐水中孵育时，平均93.6%的蠕虫从琼脂 - 内容物或琼脂 - 黏膜迁移至生理盐水中。一批琼脂的最低适宜浓度为0.85% - 0.90%，另一批为0.65%。虽然大多数蠕虫在孵育3 - 4小时后已从琼脂凝胶中迁移，但迁移通常会持续超过7小时。低浓度福尔马林会部分抑制从凝胶中的迁移，从含1%胆汁的琼脂中回收的蠕虫略多于仅含琼脂的情况。
