使用自动微生物系统从血培养物中快速自动鉴定革兰氏阴性杆菌。
Rapid automated identification of gram-negative bacilli from blood cultures with the AutoMicrobic system.
作者信息
Schifman R B, Ryan K J
出版信息
J Clin Microbiol. 1982 Feb;15(2):260-4. doi: 10.1128/jcm.15.2.260-264.1982.
Abstract
Automated identification of gram-negative bacilli directly from blood culture bottles by using the AutoMicrobic System (AMS) was evaluated with a modified procedure for the AMS Enterobacteriaceae-plus nonfermenter identification card. A total of 150 strains were tested (44 clinical and 106 seeded) and compared with a conventional identification procedure. These strains included 107 Enterobacteriaceae and 43 oxidase-positive or glucose-nonfermenting, or both, organisms. AMS identifications on one of these strains were not interpretable owing to equal probability AMS identification values. Of the remaining 149 strains, 138 (92.6%) were correctly identified within 8 to 13 h of the first reading. Of 69 identifications analyzed after 6 h of incubation, 91% were correct. This procedure was found to be rapid, convenient, and nonlabor intensive and is recommended for presumptive identification of gram-negative bacilli in blood cultures.
摘要
采用改良的自动微生物系统（AMS）肠杆菌科加非发酵菌鉴定卡程序，对直接从血培养瓶中自动鉴定革兰氏阴性杆菌进行了评估。共检测了150株菌株（44株临床菌株和106株接种菌株），并与传统鉴定程序进行了比较。这些菌株包括107株肠杆菌科细菌和43株氧化酶阳性或不发酵葡萄糖或两者兼具的细菌。由于AMS鉴定值概率相等，其中一株菌株的AMS鉴定结果无法解读。在其余149株菌株中，138株（92.6%）在首次读数后的8至13小时内被正确鉴定。在培养6小时后分析的69次鉴定中，91%是正确的。该程序快速、方便且无需大量劳动力，推荐用于血培养中革兰氏阴性杆菌的初步鉴定。
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