用于血培养细菌快速分类的直接接种程序。
Direct inoculation procedure for the rapid classification of bacteria from blood culture.
作者信息
Edberg S C, Novak M, Slater H, Singer J M
出版信息
J Clin Microbiol. 1975 Dec;2(6):469-73. doi: 10.1128/jcm.2.6.469-473.1975.
Abstract
A procedure was developed for 4-h identification of bacteria from blood culture. From a turbid blood culture bottle a 10- to 15-ml aliquot was drawn off and centrifuged. The pellet was utilized to inoculate a series of enzymatic and physiologic reactions. Three hundred eight positive blood cultures were tested including 222 strains of Enterobacteriaceae, 40 strains of facultative grampositive cocci, 26 strains of anaerobes, and 20 assorted strains. There was over 96.5% agreement between 4-h and conventional methods with the Enterobacteriaceae, 98% with facultative gram-positive cocci, 100% with anaerobes, and 99% with the assorted strains.
摘要
开发了一种用于从血培养物中4小时鉴定细菌的方法。从浑浊的血培养瓶中抽取10至15毫升等分试样并离心。沉淀用于接种一系列酶促和生理反应。对308份阳性血培养物进行了检测，包括222株肠杆菌科细菌、40株兼性革兰氏阳性球菌、26株厌氧菌和20株其他菌株。肠杆菌科细菌4小时鉴定法与传统方法的一致性超过96.5%，兼性革兰氏阳性球菌为98%，厌氧菌为100%，其他菌株为99%。
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