Nagahata T, Kiyoshige T, Tomono S, Abe R, Sasaki S, Takazoe I
Infect Immun. 1982 Apr;36(1):304-9. doi: 10.1128/iai.36.1.304-309.1982.
Oral implantation of Bacteroides asaccharolyticus 381-R' and Eikenella corrodens 1073S-R, which are highly resistant to streptomycin, was examined in conventional hamsters. The hamsters' first molars were ligatured with cotton threads preimmersed in bacterial suspensions. Bacterial inoculation was performed daily for 1 week, followed by a single weekly inoculation for 7 more weeks. Hamsters were fed Keyes' diet no. 2000 or ordinary powdered diet. Bacterial recovery, gingival histological changes, and immunological response were checked 2, 5, and 8 weeks after the daily serial inoculation. B. asaccharolyticus 381-R' was recovered from all hamsters during the experiment (6.7 X 10(6) to 6.3 X 10(8) colony-forming units). E. corrodens 10735-R was recovered from the hamsters fed Keyes' diet no. 2000 throughout the experimental period (2.4 X 10(5) to 1.2 X 10(6) colony-forming units), but not in the group fed the ordinary powdered diet. The histological changes in gingival tissues at the second week showed no differences among the control group, the B. asaccharolyticus-inoculated group, and the E. corrodens-inoculated group. However, by the eighth week, the inflammation persisted only in the bacteria-inoculated groups, especially in the B. asaccharolyticus-inoculated group. A relatively increased serum antibody titer was also observed in the B. asaccharolyticus-inoculated group, but not in the E. corrodens-inoculated group. These findings indicate that B. asaccharolyticus 381-R' and E. corrodens 1073S-R can be implanted in hamsters' oral cavity with the aid of cotton thread ligature. It was also suggested that B. asaccharolyticus may have some pathogenic role in the destruction of periodontal tissue in hamsters.
在常规仓鼠中检测了对链霉素高度耐药的解糖梭杆菌381-R'和腐蚀艾肯菌1073S-R的口腔植入情况。用预先浸泡在细菌悬液中的棉线结扎仓鼠的第一磨牙。每天进行细菌接种，持续1周，随后每周接种1次，再持续7周。仓鼠喂食凯斯2000号饮食或普通粉状饮食。在每日连续接种后的第2、5和8周检查细菌回收情况、牙龈组织学变化和免疫反应。在实验期间，所有仓鼠都检测到了解糖梭杆菌381-R'（6.7×10⁶至6.3×10⁸菌落形成单位）。在整个实验期间，喂食凯斯2000号饮食的仓鼠中检测到了腐蚀艾肯菌10735-R（2.4×10⁵至1.2×10⁶菌落形成单位），但在喂食普通粉状饮食的组中未检测到。第二周时，对照组、接种解糖梭杆菌的组和接种腐蚀艾肯菌的组之间牙龈组织学变化没有差异。然而，到第八周时，炎症仅在接种细菌的组中持续存在，尤其是在接种解糖梭杆菌的组中。在接种解糖梭杆菌的组中也观察到血清抗体滴度相对升高，但在接种腐蚀艾肯菌的组中未观察到。这些发现表明，借助棉线结扎，解糖梭杆菌381-R'和腐蚀艾肯菌1073S-R可以植入仓鼠口腔。还表明解糖梭杆菌可能在仓鼠牙周组织破坏中具有某种致病作用。
