Ahmed A G, Bremner R, Nisbet A, Horne C H, Klopper A
Clin Chim Acta. 1982 May 20;121(2):217-24. doi: 10.1016/0009-8981(82)90061-4.
Mixtures containing a fixed amount of SP1 alpha, increasing amounts of SP1 beta and vice versa have been measured by rocket immunoelectrophoresis, by enzyme immunoassay and by radioimmunoassay. Both proteins have immunological determinants which react with standard antisera to SP1 and the value obtained for any particular mixture is dependent on the ratio of the proteins. The effect of the ratio on measurement by immunoelectrophoresis is different from that on measurement by enzyme immunoassay or radioimmunoassay. As these two proteins exist together in the blood of pregnant women, Results obtained by the two types of assay cannot be compared. Even with the same method the results will depend on the ratio which varies from subject to subject. It is concluded that until specific measurements for SP1 alpha and SP1 beta are designed, clinical application studies must be viewed with reserve.
通过火箭免疫电泳、酶免疫测定法和放射免疫测定法对含有固定量的SP1α、不断增加量的SP1β以及反之亦然的混合物进行了测量。这两种蛋白质都具有免疫决定簇，可与针对SP1的标准抗血清发生反应，并且任何特定混合物所获得的值取决于蛋白质的比例。该比例对免疫电泳测量的影响不同于对酶免疫测定法或放射免疫测定法测量的影响。由于这两种蛋白质共同存在于孕妇血液中，因此这两种测定方法所获得的结果无法进行比较。即使使用相同的方法，结果也将取决于个体之间变化的比例。得出的结论是，在设计出针对SP1α和SP1β的特定测量方法之前，对临床应用研究必须持保留态度。
