Murakami K, Taniguchi H, Baba S
Diabetologia. 1982 May;22(5):358-61. doi: 10.1007/BF00253582.
Extracts from homogenates of rat and human parotid glands contained insulin-like immunoreactivity. The values were 5.6 +/- 2.8 ng/g wet tissue in six groups of rat parotid glands and 23.8 and 39.7 ng/g wet tissue in two human extracts. Upon gel filtration immunoreactive insulin of rat origin was eluted in a peak corresponding to the elution volume of isotopically labelled insulin. The material obtained from the two peak fractions showed an immunoassay dilution curve identical with that of rat insulin. Furthermore, biosynthesis of insulin-like immunoreactivity in rat and human parotid glands was confirmed in vitro by a specific separation method using anti-insulin antibody. These findings suggest that the parotid gland may be a further extrapancreatic source of insulin, and that insulin biosynthesis does occur in extrapancreatic tissues.
大鼠和人类腮腺匀浆提取物中含有胰岛素样免疫活性物质。六组大鼠腮腺中该物质的含量为5.6±2.8 ng/g湿组织，两份人类提取物中的含量分别为23.8 ng/g湿组织和39.7 ng/g湿组织。经凝胶过滤后，大鼠来源的免疫反应性胰岛素在与同位素标记胰岛素洗脱体积相对应的峰中被洗脱出来。从两个峰级分中获得的物质显示出与大鼠胰岛素相同的免疫测定稀释曲线。此外，通过使用抗胰岛素抗体的特异性分离方法在体外证实了大鼠和人类腮腺中胰岛素样免疫活性物质的生物合成。这些发现表明，腮腺可能是胰岛素的另一个胰腺外来源，并且胰岛素生物合成确实发生在胰腺外组织中。
