Farram E, Nelson M, Nelson D S, Moon D K
Immunology. 1982 Jul;46(3):603-12.
Supernatants from cultured mouse and human tumour cells, but not mouse or guinea-pig fibroblasts, inhibited the production of a lymphokine, macrophage chemotactic factor, by PHA-stimulated mouse spleen cells. The supernatants affected spleen cells from old, but not young, mice. They were most active if added at the start of the spleen cell culture and did not act by binding phytohaemagglutinin (PHA). The active material had an approximate molecular weight, on membrane filtration, of 1000-10,000 and could be bound to and eluted from Con A-Sepharose. Tumour supernatant factor(s) of similar molecular weight inhibited the production of interleukin 1 (lymphocyte activating factor) in response to lipopolysaccharide by stimulated thioglycollate-induced peritoneal exudate macrophages, but not by Corynebacterium parvum-activated macrophages. Similar tumour-produced material has been found to inhibit the early phase of delayed-type hypersensitivity reactions in older mice. It is suggested that this effect is due, at least in part, to inhibition of interleukin 1 production leading to inhibition of lymphokine production.
培养的小鼠和人类肿瘤细胞的上清液，而非小鼠或豚鼠成纤维细胞的上清液，可抑制PHA刺激的小鼠脾细胞产生一种淋巴因子——巨噬细胞趋化因子。这些上清液对老年小鼠的脾细胞有影响，对幼年小鼠的脾细胞则无影响。若在脾细胞培养开始时添加，它们的活性最强，且并非通过结合植物血凝素（PHA）起作用。经膜过滤，活性物质的分子量约为1000 - 10000，可与伴刀豆球蛋白A - 琼脂糖珠结合并洗脱。分子量相似的肿瘤上清液因子可抑制经刺激的巯基乙酸盐诱导的腹腔渗出巨噬细胞对脂多糖产生白细胞介素1（淋巴细胞激活因子），但对短小棒状杆菌激活的巨噬细胞无此作用。已发现类似的肿瘤产生物质可抑制老年小鼠迟发型超敏反应的早期阶段。提示这种作用至少部分是由于抑制白细胞介素1的产生，进而导致淋巴因子产生受到抑制。
