Kindblom L G, Seidal T, Karlsson K
Acta Pathol Microbiol Immunol Scand A. 1982 May;90(3):167-74. doi: 10.1111/j.1699-0463.1982.tb00078_90a.x.
An immuno-histochemical investigation of the presence and localization of myoglobin was performed on cardiac and skeletal muscle tissue and on 9 embryonal and 9 alveolar rhabdomyosarcomas, utilizing an immunoperoxidase technique. Cardiac muscle fibres were evenly stained whereas staining of skeletal muscle fibres varied, giving a mosaic-like pattern. Of the fixatives used (4 percent formaldehyde, 4 percent paraformaldehyde, formaldehyde-glutaraldehyde, and Bouin's fixative), 4 percent formaldehyde gave the most prominent staining; short fixation-time slightly increased the staining intensity. All the 9 embryonal and 9 alveolar rhabdomyosarcomas contained positively-stained tumour cells. The number of cells positively-stained and the intensity of the staining varied with differentiation: the most differentiated rhabdomyoblasts stained most intensely. However, also many poorly-differentiated tumour cells in highly cellular areas were positive. Myoglobin is considered a suitable marker of rhabdomyoblastic differentiation, thus the method used in this study may be valuable in the diagnosis.
利用免疫过氧化物酶技术，对心肌和骨骼肌组织以及9例胚胎性横纹肌肉瘤和9例肺泡型横纹肌肉瘤进行了肌红蛋白存在及定位的免疫组织化学研究。心肌纤维被均匀染色，而骨骼肌纤维的染色各不相同，呈现出马赛克样的图案。在所使用的固定剂（4%甲醛、4%多聚甲醛、甲醛-戊二醛和Bouin固定液）中，4%甲醛染色最为明显；短时间固定可略微增加染色强度。9例胚胎性横纹肌肉瘤和9例肺泡型横纹肌肉瘤均含有阳性染色的肿瘤细胞。阳性染色细胞的数量和染色强度随分化程度而异：分化程度最高的成肌细胞染色最强。然而，在细胞高度密集区域的许多低分化肿瘤细胞也呈阳性。肌红蛋白被认为是成肌细胞分化的合适标志物，因此本研究中使用的方法在诊断中可能具有价值。
