使用庆大霉素酶免疫测定法测定奈替米星。
Assay of netilmicin, using enzyme immunoassay for gentamicin.
作者信息
Larson T, Gerding D N, Peterson L R, Eckfeldt J H
出版信息
Antimicrob Agents Chemother. 1982 Mar;21(3):399-401. doi: 10.1128/AAC.21.3.399.
Abstract
A homogenous enzyme immunoassay for the quantitative determination of netilmicin in serum was developed. The procedure utilizes a commercially available assay for gentamicin (EMIT; Syva Co., Palo Alto, Calif.). The method was adapted to a microcentrifugal analyzer, and log-logit regression analysis was performed with a computer. The results of samples assayed by this method correlate well with microbioassay (r2 = 0.985) and radioimmunoassay (r2 = 0.986). This method is not only precise and accurate, but also very rapid and economical and compares favorably to other available methods of netilmicin assay.
摘要
开发了一种用于定量测定血清中奈替米星的均相酶免疫测定法。该方法利用市售的庆大霉素测定法（EMIT；Syva公司，加利福尼亚州帕洛阿尔托）。该方法适用于微量离心分析仪，并使用计算机进行对数-对数逻辑回归分析。用该方法测定的样品结果与微生物测定法（r2 = 0.985）和放射免疫测定法（r2 = 0.986）相关性良好。该方法不仅精确、准确，而且非常快速、经济，与其他现有的奈替米星测定方法相比具有优势。
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Antimicrob Agents Chemother. 1982 Mar;21(3):399-401. doi: 10.1128/AAC.21.3.399.
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本文引用的文献
1
Comparison of five methods for the assay of serum gentamicin.血清庆大霉素测定五种方法的比较
Antimicrob Agents Chemother. 1981 Apr;19(4):508-12. doi: 10.1128/AAC.19.4.508.
2
Evaluation of five gentamicin assay procedures for clinical microbiology laboratories.临床微生物学实验室五种庆大霉素检测方法的评估
J Clin Microbiol. 1981 Apr;13(4):742-9. doi: 10.1128/jcm.13.4.742-749.1981.
3
Disc plate method of microbiological antibiotic assay. I. Factors influencing variability and error.微生物抗生素测定的纸片法。I. 影响变异性和误差的因素。
Appl Microbiol. 1971 Oct;22(4):659-65. doi: 10.1128/am.22.4.659-665.1971.
4
Comparative nephrotoxicities of netilmicin and gentamicin in rats.奈替米星和庆大霉素对大鼠的肾毒性比较
Antimicrob Agents Chemother. 1976 Nov;10(5):845-9. doi: 10.1128/AAC.10.5.845.
5
Radioimmunoassay of sisomicin.西索米星的放射免疫测定法。
Antimicrob Agents Chemother. 1976 Feb;9(2):247-50. doi: 10.1128/AAC.9.2.247.
6
High-pressure liquid chromatographic assay of netilmicin in plasma.血浆中奈替米星的高压液相色谱测定法。
Antimicrob Agents Chemother. 1977 Dec;12(6):707-9. doi: 10.1128/AAC.12.6.707.
7
125I radioimmunoassay of netilmicin.奈替米星的125I放射免疫测定法
Antimicrob Agents Chemother. 1977 Apr;11(4):768-70. doi: 10.1128/AAC.11.4.768.
8
Netilmicin and gentamicin: comparative pharmacology in humans.奈替米星与庆大霉素：人体中的比较药理学
Antimicrob Agents Chemother. 1977 Apr;11(4):609-14. doi: 10.1128/AAC.11.4.609.
9
In vitro activity of netilmicin.奈替米星的体外活性。
Antimicrob Agents Chemother. 1977 Feb;11(2):362-4. doi: 10.1128/AAC.11.2.362.
10
Antimicrobial activity in vitro of netilmicin and comparison with sisomicin, gentamicin, and tobramycin.奈替米星的体外抗菌活性及其与西索米星、庆大霉素和妥布霉素的比较。
Antimicrob Agents Chemother. 1977 Jan;11(1):118-21. doi: 10.1128/AAC.11.1.118.
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