Vandvik B, Nilsen R E, Vartdal F, Norrby E
Acta Neurol Scand. 1982 May;65(5):468-87. doi: 10.1111/j.1600-0404.1982.tb03104.x.
Intrathecal production of oligoclonal mumps-specific IgG was demonstrated in nine out of 10 children with mumps meningitis by imprint immunofixation (IIF) of sera and cerebrospinal fluids (CSF) separated by agarose electropheresis and by thin-layer electrofocusing. Four of the patients had intrathecal mumps antibody synthesis demonstrable also by conventional serological tests. Oligoclonal CSF IgG was demonstrable by agarose electrophoresis in four of the patients. A dominance of lambda over k type oligoclonal Ig and mumps antibodies was observed in the CSF of three of these patients. The bulk of the oligoclonal CSF IgG was concluded to represent mumps-specific antibodies on the basis of the IIF as well as virus absorption analysis. Intrathecal production of oligoclonal IgG antibodies to one, two, or three other (measles, rubella, herpes simplex) viruses was demonstrated by IIF in four patients. These antibodies were not associated with the oligoclonal CSF IgG present in three of the patients. It is concluded that a specific intrathecal IgG antibody response is a common feature in children with mumps meningitis. This response sometimes reaches a magnitude that permits detection of oligoclonal IgG in the CSF. In some patients, the specific response appears to be associated with a non-specific activation of cells producing antibodies of other (unrelated) specificity.
通过琼脂糖电泳和薄层电聚焦分离血清和脑脊液（CSF），并进行印迹免疫固定（IIF），在10例腮腺炎脑膜炎患儿中的9例中证实了鞘内产生寡克隆腮腺炎特异性IgG。其中4例患者通过传统血清学检测也可证明有鞘内腮腺炎抗体合成。4例患者通过琼脂糖电泳可检测到寡克隆CSF IgG。在其中3例患者的CSF中观察到λ型寡克隆Ig和腮腺炎抗体占主导地位。基于IIF以及病毒吸收分析，得出结论：大部分寡克隆CSF IgG代表腮腺炎特异性抗体。通过IIF在4例患者中证实了鞘内产生针对一种、两种或三种其他（麻疹、风疹、单纯疱疹）病毒的寡克隆IgG抗体。这些抗体与3例患者中存在的寡克隆CSF IgG无关。得出结论：特异性鞘内IgG抗体反应是腮腺炎脑膜炎患儿的常见特征。这种反应有时达到可在CSF中检测到寡克隆IgG的程度。在一些患者中，特异性反应似乎与产生其他（不相关）特异性抗体的细胞的非特异性激活有关。
