从人类粪便中分离出链球菌。
The isolation of streptococci from human faeces.
作者信息
Unsworth P F
出版信息
J Hyg (Lond). 1980 Aug;85(1):153-64. doi: 10.1017/s0022172400027169.
Three selective media were designed for isolation of streptococci from faeces. Samples of faeces from twelve normal adults were suspended and serially diluted in saline or broth saline, and equal volumes of each dilution were spread and incubated on the media. The number of colonies of each different type which developed was counted and one colony of each type was subcultured and identified. Altogether, streptococci of 13 taxa were found. S. faecium, S. mutans, S. milleri, S. faecalis and S. mitior were each found in over half the samples. Lancefield group B and G streptococci, S. bovis II, an atypical strain of S. bovis I, S. cremoris, S. durans and a dextran-positive strain of S. mitior were each present in 1 or 2 samples. Individual samples contained 2-7 (mean 4.6) streptococcal taxa, and total viable counts of streptococci of 3 X 10(3)-3 X 10(8) (geometric mean 7 X 10(5)) per g. The significance of these findings is discussed.
设计了三种选择性培养基用于从粪便中分离链球菌。将12名正常成年人的粪便样本悬浮于生理盐水或肉汤盐水中并进行系列稀释，然后将各稀释度的等体积样本涂布于培养基上并进行培养。对生长出的每种不同类型的菌落进行计数，并对每种类型的一个菌落进行传代培养和鉴定。总共发现了13个分类单元的链球菌。在超过半数的样本中均发现了屎肠球菌、变形链球菌、米勒链球菌、粪肠球菌和缓症链球菌。B群和G群兰斯菲尔德链球菌、牛链球菌II型、牛链球菌I型的一株非典型菌株、嗜热链球菌、耐久链球菌以及缓症链球菌的一株葡聚糖阳性菌株分别出现在1或2个样本中。单个样本含有2 - 7种（平均4.6种）链球菌分类单元，每克粪便中链球菌的总活菌数为3×10³ - 3×10⁸（几何平均数为7×10⁵）。讨论了这些发现的意义。
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