Goldbaum M H, Madden K
Br J Ophthalmol. 1982 Jan;66(1):17-25. doi: 10.1136/bjo.66.1.17.
Trypsin digestion of retinal pigment epithelium is a technique that bares Bruch's membrane, to allow topographical examination by scanning electron microscopy. Twenty-five human eyes were examined. The zonula occludens of the pigment epithelium was clearly seen as a surface feature, but attachment plaques at the sides and base were not visible. The adhesion between the pigment epithelium and the basal lamina was stronger than between the basal lamina and the rest of Bruch's membrane. Surface features of the basal lamina, inner collagenous zone, elastic layer, and outer collagenous zone were seen in a way that previously required an artist's representation constructed from microscopic sections.
视网膜色素上皮的胰蛋白酶消化是一种暴露布鲁赫膜的技术，以便通过扫描电子显微镜进行地形学检查。对25只人眼进行了检查。色素上皮的紧密连接作为一种表面特征清晰可见，但侧面和底部的附着斑不可见。色素上皮与基膜之间的黏附力比基膜与布鲁赫膜其余部分之间的黏附力更强。基膜、内胶原区、弹性层和外胶原区的表面特征以一种以前需要根据显微切片构建的艺术家描绘的方式呈现出来。
