Mountford C E, Grossman G, Hampson A W, Holmes K T
Biochim Biophys Acta. 1982 Feb 10;720(1):65-74. doi: 10.1016/0167-4889(82)90039-8.
High resolution 1H-NMR spectroscopy has been used to study the infection of chicken embryo fibroblasts by influenza virus. Marked changes in the NMR spectrum occur when infectious influenza virus is introduced into the fibroblasts and these changes appear to depend upon the presence of active neuraminidase (EC 3.2.1.18). A crude preparation of neuraminidase from Vibrio cholerae is able to effect similar changes. Only minor spectral changes are observed in the absence of culture medium or when the viral genome material is inactivated by beta-propiolactone. Similarly, little change is seen in the NMR spectrum when amantadine, which is thought to inhibit uncoating of the virus inside the cell, or actinomycin D, which inhibits cellular nucleic acid metabolism, are incubated with fibroblasts prior to the addition of virus. The results suggest that neuraminidase, in co-operation with a factor in the infectious process, initiates a cellular event which can be monitored by NMR. The nature of this cellular mechanism is unknown, but further studies are under way to determine its importance in viral infection.
高分辨率1H-NMR光谱已被用于研究流感病毒对鸡胚成纤维细胞的感染。当将具有感染性的流感病毒引入成纤维细胞时，NMR光谱会发生显著变化，并且这些变化似乎取决于活性神经氨酸酶（EC 3.2.1.18）的存在。霍乱弧菌神经氨酸酶的粗制品能够产生类似的变化。在没有培养基的情况下，或者当病毒基因组物质被β-丙内酯灭活时，仅观察到微小的光谱变化。同样，当在添加病毒之前将被认为可抑制病毒在细胞内脱壳的金刚烷胺或抑制细胞核酸代谢的放线菌素D与成纤维细胞一起孵育时，NMR光谱中也几乎没有变化。结果表明，神经氨酸酶与感染过程中的一个因子协同作用，引发了一个可以通过NMR监测的细胞事件。这种细胞机制的本质尚不清楚，但正在进行进一步的研究以确定其在病毒感染中的重要性。
