单链RNA中未配对碱基自由能分配的微小变化不会影响预测的二级结构。
Small changes in free energy assignments for unpaired bases do not affect predicted secondary structures in single stranded RNA.
作者信息
Nussinov R, Tinoco I, Jacobson A B
出版信息
Nucleic Acids Res. 1982 Jan 11;10(1):341-9. doi: 10.1093/nar/10.1.341.
Abstract
We present extensive calculations of the secondary structure of mRNA which point to its insensitivity to small changes in the free energy assignments of single stranded regions. Truncating the free energies of hairpin loops, bulges, internal loops and multibranched junctions to two significant digits yields structures nearly identical to those generated using three digit values. The results show that one can safely use truncated values in RNA folding calculations. The implementation of these results enabled us to carry out secondary structure calculations on 2600 nucleotides in a single computer run.
摘要
我们展示了对信使核糖核酸（mRNA）二级结构的广泛计算，这些计算表明其对单链区域自由能分配的微小变化不敏感。将发夹环、凸起、内环和多分支连接的自由能截断为两位有效数字，得到的结构与使用三位数字值生成的结构几乎相同。结果表明，在RNA折叠计算中可以安全地使用截断值。这些结果的应用使我们能够在一次计算机运行中对2600个核苷酸进行二级结构计算。
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本文引用的文献
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