Jakobsen J, Jensen H D, Sidenius P
Acta Neuropathol. 1982;56(2):157-60. doi: 10.1007/BF00690588.
Mast cell proliferation has been reported in peripheral nerves of streptozotocin diabetic rats. We examined the question by light microscopy using morphometric techniques including estimation of shape and size. After 4 weeks of diabetes the mast cell area in transverse sections was 32.7 +/- 7.0 microns 2 vs. 22.5 +/- 5.4 microns 2 in controls (P less than 10(2)). Mast cell profiles approximated to ellipses in longitudinal sections, the long diameter being 11.4 +/- 1.2 microns vs. 14.0 +/- 1.0 microns in controls (P less than 10(4)). Correspondingly, the profile number was increased (30 +/- 6%) in longitudinal sections and decreased in transverse sections (11 +/- 16%). The present study does not provide evidence for mast cell proliferation in peripheral nerves in experimental diabetes, and, furthermore, underlines the need of evaluation of dimensions in studies of particle numbers.
据报道，链脲佐菌素诱导的糖尿病大鼠外周神经中存在肥大细胞增殖。我们使用形态计量学技术，包括形状和大小估计，通过光学显微镜检查了这个问题。糖尿病4周后，横切面上肥大细胞面积为32.7±7.0平方微米，而对照组为22.5±5.4平方微米（P<10⁻²）。在纵切面上，肥大细胞轮廓近似椭圆形，长径为11.4±1.2微米，而对照组为14.0±1.0微米（P<10⁻⁴）。相应地，纵切面上轮廓数量增加（30±6%），横切面上减少（11±16%）。本研究没有提供实验性糖尿病外周神经中肥大细胞增殖的证据，此外，强调了在颗粒数量研究中评估尺寸的必要性。
