Trautwetter A, Ritzenthaler P, Alatossava T, Mata-Gilsinger M
J Virol. 1986 Sep;59(3):551-5. doi: 10.1128/JVI.59.3.551-555.1986.
Bacteriophage LL-H is a virulent phage of Lactobacillus lactis LL23. A restriction map of the phage genome was constructed with various restriction endonucleases. This chromosome has a 34-kilobase size and seems to be circularly permuted. We used a bank of LL-H restriction fragments to study the expression of five of the seven main phage particle proteins. Immunoblotting experiments permitted the mapping on the chromosome of several genes coding for phage particle proteins. We also show that the gene of the main capsid protein is expressed from its own promoter in an Escherichia coli strain.
噬菌体LL-H是乳酸乳球菌LL23的烈性噬菌体。用多种限制性核酸内切酶构建了该噬菌体基因组的限制图谱。该染色体大小为34千碱基，似乎是环状排列的。我们利用一组LL-H限制片段研究了七种主要噬菌体颗粒蛋白中五种的表达情况。免疫印迹实验允许在染色体上定位几个编码噬菌体颗粒蛋白的基因。我们还表明，主要衣壳蛋白的基因在大肠杆菌菌株中从其自身启动子表达。
