Clarke C, Dawson K B, Sheldon P W
Br J Cancer. 1982 Apr;45(4):582-7. doi: 10.1038/bjc.1982.95.
A quantitative, cytochemical assay for measuring lysosomal enzymes in the peripheral nerves of mice has been developed. That the time course of lysosomal enzyme changes after misonidazole (MISO) treatment reflects the degree of neurotoxicity of this agent in the mouse, has been confirmed by the use of two known neurotoxic compounds: methyl mercury and acrylamide. This effect is specific to the peripheral nerves and was not found in liver, kidney, heart or cerebral cortex. Enzyme activities varied with mouse strain and sex, as did the response to MISO treatment. Of the mice studied, female C57 gave the greatest increase in beta-glucuronidase activity. With the MISO dose of 0.6 mg/g/dose the increased enzyme activity was independent of the route of administration and appeared to approach a plateau after 5 daily doses.
已开发出一种用于测量小鼠外周神经中溶酶体酶的定量细胞化学测定法。通过使用两种已知的神经毒性化合物：甲基汞和丙烯酰胺，已证实米索硝唑（MISO）治疗后溶酶体酶变化的时间进程反映了该药物在小鼠中的神经毒性程度。这种效应对外周神经具有特异性，在肝脏、肾脏、心脏或大脑皮层中未发现。酶活性随小鼠品系和性别而变化，对MISO治疗的反应也是如此。在所研究的小鼠中，雌性C57小鼠的β-葡萄糖醛酸酶活性增加最大。在MISO剂量为0.6 mg/g/剂量时，酶活性的增加与给药途径无关，并且在每日给药5次后似乎接近平台期。
